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Enzyme Substrates

Targets that are detected with an enzyme conjugate can be visualized with an appropriate enzyme substrate that produces a chemiluminescent/chemifluorescent or chromogenic reaction product.

The DuoLuX™ Chemiluminescent/Chemifluorescent Substrate is a unique formula based on acridan chemistry that produces both a strong chemiluminescent and fluorescent signal. The half-life of luminescent emission exceeds that of many other luminescent substrates.

Chromogenic substrates can also be used to visualize targets detected with enzyme-based systems. The substrates deposit a colored precipitate directly on the membrane, eliminating the additional steps of exposing and developing film, or image capture. The variety of chromogenic substrates also allows multiple targets to be visualized simultaneously on the blot.

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