Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

Western Blot Blocking Solutions

In dot blot or western blot application, blocking solutions minimize the non-specific binding of detection antibodies to the membrane that lead to background.

Vector Laboratories offers two solutions formulated to block membranes in blotting applications:

  • WestVision™ Block and Diluent — a proprietary, ready-to-use formulation containing Tween 20 that improves signal intensity and resolution without increasing background. It is compatible with both alkaline phosphatase- and peroxidase- based detections systems, and with both chemiluminescence and chromogenic development.
  • Animal-Free Blocker™ — a plant-derived universal antibody diluent and blocking reagent for nucleic acid and protein blotting, IHC, and for other applications. It contains no animal-derived protein and can be used as an alternative to sera, BSA, casein, or non-fat dry milk.
SP 7000 1600 4 2
4-fold serial dilutions of human recovered plasma were transferred to nitrocellulose. Membranes were blocked with WestVistionTM Block & Diluent (A) or Competitor Block (B), probed with Rabbit anti-Transferrin antibody (0.25 ug/ml), and detected with WestV