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AZDye 660 NHS Ester (FP-1821)

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Description

AZDye 660 is a bright and photostable near-IR dye that is spectrally almost identical to Alexa Fluor® 660 and CF660® 680RD. AZDye 680 is often used for a bright and photostable far-red dye with excitation ideally suited to the 633 or 647 nm laser line. AZDye 660 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 660 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.

The NHS ester (succinimidyl ester) of AZDye 660 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima662/650 nm
Extinction coefficient250,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 660, CF® 660
Molecular weight1089.16
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF660

Protein Labeling Calculator

[protein-labeling-calculator]

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