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AZDye 594 Tyramide (CCT-1542)

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Description

Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 594 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar DyesAlexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight841.95 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceDark blue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF594

Applicable patents and legal notices are available at legal notices.

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