Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

AZDye 594 TFP Ester (FP-1111)

[text-backorder]

This product was previously sold as 1433 from Click Chemistry Tools.

Description

AZDye™ 594 TFP Ester is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye often used for modifying proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules with AZDye™ 594 label. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. AZDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

TFP (tetrafluorophenyl) ester is an amine-reactive activated ester that reacts with primary amines of biomolecules in the same way as the succinimidyl ester (SE or NHS-ester) to form a stable amide bond. The major advantage of TFP esters over the succinimidyl ester is much improved resistance to spontaneous hydrolysis during conjugation reactions resulting in more efficiency and better reproducible labeling of biopolymers. TFP esters are stable for several hours at the basic pH typically used for reactions–far outlasting succinimidyl esters.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima590/617 nm
Extinction coefficient92,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 594, CF™ 594, DyLight® 594
Molecular weight870.84
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF594

Protein Labeling Calculator

[protein-labeling-calculator]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote