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AZDye 555 Picolyl Azide (CCT-1288)

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This product was previously sold as 1171 from Fluoroprobes.

Description

AZDye™ 555 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AZDye™ 555 Picolyl Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight1049.22 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF555

Applicable patents and legal notices are available at legal notices.

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