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AZDye 488 Acid (FP-1012)

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Description

AZDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AZDye™ 488 is green-emitting dye with excitation ideally suited for the 488 nm line. Its fluorescence is pH independent from pH 4 to pH 10.

AZDye™ 488 Acid often used for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), and STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for the non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, e.g. HATU.

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amines
Abs/Em Maxima494/517 nm
Extinction coefficient73,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight534.47 (protonated)
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Applicable patents and legal notices are available at legal notices.

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