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AZDye 430 NHS Ester (FP-1031)

Description

AZDye™ 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityAlkynes, cyclooctynes
Abs/Em Maxima430/539 nm
Extinction coefficient15,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 430
Molecular weight600.56
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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Protein Labeling Calculator

[protein-labeling-calculator]

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