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AZDye 405 Gly-Gly-Gly (CCT-1583)

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Description

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”.

AZDye™ 405 is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima405/425nm
Extinction Coefficient
35,000
Spectrally Similar DyesAlexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight889.92
SolubilityWater, DMSO, DMF
AppearanceYellow to grey solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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