AZDye 405 Alkyne

AZDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

Price range: $187.00 through $1,524.00

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SKU: CCT-1309
CAS Number
N/A
Molecular Weight
553.53 (protonated), 619.47 (sodium salt)
Appearance
Yellow solid
Extinction Coefficient
35,000
Purity
>95% (HPLC)
Unit Size
1 mg, 5 mg, 25 mg
Solubility
Water, DMSO, DMF
Storage Instructions
-20°C. Desiccate
Spectrally Similar Dyes
Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Laser Line
405 nm
Excitation/Emission Maximum
402/424 nm
Shipping Conditions
Ambient temperature
Shipping Instructions
Ambient temperature
Abs/Em Spectra

Af405

Selected References
  1. Costello, A., et al. (2021). Directed evolution of rRNA improves translation kinetics and recombinant protein yield. Nat Commun., 12 (1), 5638. [PubMed]