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AZDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.
AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.
AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.
DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 402/424 nm |
| Extinction Coefficient | 35,000 |
| Flow Cytometry Laser Line | 405 nm |
| Microscopy Laser Line | 405 nm |
| Spectrally Similar Dyes | Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405 |
| Molecular weight | 553.53 (protonated), 619.47 (sodium salt) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Yellow solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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