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Amino-dPEG®₈-t-butyl ester (QBD-10271)



Amino-dPEG®8–t-butyl ester, product number QBD-10271, is a carbonyl- and amine-reactive, single molecular weight polyethylene glycol (PEG) product with a discrete chain length. The compound consists of a primary amine and a propionic acid tert-butyl ester on opposite ends of a discrete PEG (dPEG®) spacer. The amine end of the molecule reacts readily with aldehydes and ketones to form reducible Schiff bases and with carboxylic acids and their active esters (NHS, TFP) to form amide bonds. A tert-butyl ester group protects the propionic acid end of the product. The ester is cleavable with trifluoroacetic acid (TFA), leaving the propionic acid moiety free to react with amines either by activation with an acylation reagent or by direct coupling to an amine using a carbodiimide.

This product can be used in peptide synthesis, protein modification, other forms of supramolecular construction, and as a monoprotected heterobifunctional crosslinker. The dPEG® spacer is not a dispersed polymer with a range of chain lengths and molecular weights but a very high purity, single molecular weight PEG chain with a discrete chain length. One of the advantages of dPEG® compounds is that analyses of conjugates made with dPEG® linkers are simplified since only one chain length and molecular weight of PEG is present. Moreover, our amphiphilic dPEG® products impart water solubility to conjugates made with them, often improving the performance of the conjugate compared to the parent molecule.


Unit Size100 mg, 1000 mg
Molecular Weight497.62; single compound
Chemical formulaC₂₃H₄₇NO₁₀
Purity> 98%
SpacersdPEG® Spacer is 28 atoms and 32.2 Å
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, or DMSO .
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.


Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

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Optimization of solid phase PLA with emphasis on antibody modification. Junhong Yan. Thesis. Uppsala university, Uppsala, Sweden. 2010

Biomolecular strategies for cell surface engineering. John Tanner Wilson. Doctoral Dissertation, Georgia Institute of Technology: Atlanta, Georgia USA, 2009.

Native Polycystin 2 Functions as a Plasma Channel in Renal Epithelia Membrane Ca 2+-Permeable Cation Channel in Renal Epithelia.Ying Luo, Peter M. Vassilev, Xiaogang Li, Yoshifumi Kawanabe and Jing Zhou. Molecular and Cellular Biology. 2003, 23(7) pp. 2600-2607. April 1, 2003. DOI:10.1128/MCB.23.7.2600-2607.

Molecular Cloning, Functional Expression, and Tissue Distribution of a Novel Human Gap Junction-forming Protein, Connexin-31.9: Interaction With Zona Occludens Protein-1. Peter A. Nielsen, Derek L. Beahm, Ben N. G. Giepmans, Amos Baruch, James E. Hall and Nalin M Kumar. The Journal of Biological Chemistry. 2002, 277 (41) pp. 38272-38283. August 1, 2002. DOI: 10.1074/jbc.M205348200.

An analysis of the function and expression of D6 on lymphatic endothelial cells. Clive S. McKimmie, Mark D. Singh, Kay Hewit, Oscar Lopez-Franco, Michelle Le Brocq, Stefan Rose-John, Kit Ming Lee, Andrew H. Baker, Rachel Wheat, David J. Blackbourn, Robert J. B. Nibbs and Gerard J. Graham. Blood Journal. 2013, 121 (18), pp 3768-3777. May 2, 2013. DOI: 10.1182/blood-2012-04-425314.

Induction of Terminal Differentiation in Melanoma Cells on Downregulation of b-Amyloid Precursor Protein. Michelle G. Botelho, Xiaolei Wang, Donna J. Arndt-Jovin, Dorothea Becker and Thomas M. Jovin. Journal of Investigative Dermatology. 2010, 130, pp 1400-1410. September 17, 2009. DOI:10.1038/jid.2009.296.

Ligand-regulated oligomerization of b2-adrenoceptors in a model lipid bilayer. Juan Jose´ Fung, Xavier Deupi, Leonardo Pardo, Xiao Jie Yao, Gisselle A Velez-Ruiz, Brian T DeVree, Roger K Sunahara and Brian K Kobilka. The EMBO Journal. 2009, 28 (21) pp 3315–3328. September 17, 2009. DOI: 10.1038/emboj.2009.267.

SURFIN is a polymorphic antigen expressed on Plasmodium falciparum merozoites and infected erythrocytes. Gerhard Winter, Satoru Kawai, Malin Haeggström, Osamu Kaneko, Anne von Euler, Shin-ichiro Kawazu, Daniel Palm, Victor Fernandez, and Mats Wahlgren. JEM. 2005, 201 (11) pp 1853–1863. June 6, 2005. DOI:10.1084/jem.20041392.

Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. Soshana Svendsen, Chad Zimprich, Mark G McDougall, Dieter H Klaubert and Georgyi V Los. BMC Cell Biology. 2008, 9 (17) pp 1471. April 2, 2008. DOI: 10.1186/1471-2121-9-17.

Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. J Wykosky, E Palma, D M Gibo, S Ringler, CP Turner and W Debinski. Oncogene. 2008. 27, pp 7260–7273.September 15, 2008. DOI: 10.1038/onc.2008.328.

Recurrence of Intracranial Tumors following Adoptive T Cell Therapy Can Be Prevented by Direct and Indirect Killing Aided by High Levels of Tumor Antigen Cross-Presented on Stromal Cells. Diana L. Thomas, Miri Kim, Natalie A. Bowerman, Samanthi Narayanan, David M. Kranz, Hans Schreiber and Edward J. Roy. The Journal of Immunology. 2009, 183 (3) pp 1828-1837. July 10, 2009. DOI: 10.4049/jimmunol.0802322.

Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells. Lia Tesfay, Annissa J. Huhn, Heather Hatcher, Frank M. Torti1, Suzy V. Torti. PLoS ONE. 2012, 7 (7): e40030. July 2, 2012. DOI:10.1371/journal.pone.0040030.

Bivalent carbohydrate binding is required or biological activity of CNL, the LacdiNAc (GalNAcb1-4GlcNAc)-specific lectin from basidiomycete Clitocybe nebularis. Jure Pohleven, Miha Renko, Spela Magister, David F. Smith, Markus Kuenzler, Borut Strukelj, Dusan Turk, Janko Kos and Jerica Sabotic. The Journal of Biological Chemistry. 2012, 287 (13) pp 10602-10612. February 1, 2012. DOI:10.1074/jbc.M111.317263.

A Small-molecule Inhibitor, 5′-O-Tritylthymidine, targets FAK and Mdm-2 Interaction, and Blocks Breast and Colon Tumorigenesis in vivo. Vita Golubovskaya, Nadia L. Palma, Min Zheng, Baotran Ho, Andrew Magis, David Ostrov, and William G. Cance. Anticancer Agents Med Chem. 2013, 13 (4) pp 532–545. May 1, 2013.

A small molecule focal adhesion kinase (FAK) inhibitor, targeting Y397 site: 1-(2-hydroxyethyl) -3, 5, 7-triaza-1-azoniatricyclo [,7]decane; bromide effectively inhibits FAK autophosphorylation activity and decreases cancer cell viability, clonogenicity and tumor growth in vivo. Vita M.Golubovskaya, Sheila Figel, Baotran T.Ho, Christopher P. Johnson, Michael Yemma, Grace Huang, Min Zheng, Carl Nyberg, Andrew Magis,David A.Ostrov, Irwin H.Gelman and William G. Cance. Carcinogenesis. 2012, 33 (5) pp 1004–1013. March 7, 2012. DOI:10.1093/carcin/bgs120.

An RNAi therapeutic targeting Tmprss6 decreases iron overload in Hfe mice and ameliorates anemia and iron overload in murine b -thalassemia intermedia. Paul J. Schmidt, Iva Toudjarska, Anoop K. Sendamarai, Tim Racie, Stuart Milstein, Brian R. Bettencourt, Julia Hettinger, David Bumcrot and Mark D. Fleming. Blood Journal. 2013, 121 (7) pp 1200-1208. February 14, 2013. DOI: 10.1182/blood-2012-09-453977.

Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathway. Timothy C. Cheung, Ian R. Humphreys, Karen G. Potter, Paula S. Norris, Heather M. Shumway, Bonnie R. Tran, Ginelle Patterson, Rochelle Jean-Jacques, Miri Yoon, Patricia G. Spear, Kenneth M. Murphy, Nell S. Lurain, Chris A. Benedict, and Carl F. Ware. PNAS. 2005, 102 (37) pp 13218-13223. September 13, 2005. DOI: 10.1073/pnas.0506172102.

Epithelial Cell Retention of Transcriptionally Active, P3HR-1-Derived Heterogeneous Epstein-Barr Virus DNA with Concurrent Loss of Parental Virus. Kazufumi Ikuta, Mingyu Ding, Fangfang Zhang, John W. Sixbey and Rona S. Scott. Journal of Virology. 2011, 85 (15) pp 7634-7643. August 1, 2011. DOI: 10.1128/JVI.00045-11.

Diabetes Insipidus in Mice with a Mutation in Aquaporin-2. David J. Lloyd, Frank Wesley Hall, Lisa M. Tarantino, Nicholas Gekakis. PLoS Genetics. 2005, 1 (2) pp e20. August 19, 2005. DOI:10.1371/journal.pgen.0010020.

Development of an ELISA detecting Tumor Protein 53-Induced Nuclear Protein 1 in serum of prostate cancer patients. Houda Saadi, Marion Seillier, Maria Jose Sandi, Sylvain Peuget , Christine Kellenberger, Gwenaelle Gravi, Nelson J. Dusetti, Juan L. Iovanna, Palma Rocchi, Mohamed Amri, Alice Carrier. Results in Immunology. 2013, 3 pp 51-56. May 13, 2013. DOI: 10.1016/j.rinim.2013.05.002

Cutting Edge: A Monoclonal Antibody Specific for the Programmed Death-1 Homolog Prevents Graft-versus-Host Disease in Mouse Models. Dallas B. Flies, Shengdian Wang, Haiying Xu and Lieping Chen. The Journal of Immunology. 2011, 187 (4) pp 1537-1541. July 18, 2011. doi: 10.4049/jimmunol.1100660.

Members of RTP and REEP Gene Families Influence Functional Bitter Taste Receptor Expression. Maik Behrens, Juliane Bartelt, Claudia Reichling, Marcel Winnig, Christina Kuhn, and Wolfgang Meyerhof. The Journal of Biological Chemistry. 2006, 281 (29) pp 20650–20659. July 21, 2006. DOI:10.1074/jbc.M513637200.

Immune response to a potyvirus with exposed amino groups available for chemical conjugation. Carlos Alberto Manuel-Cabrera, Ana Márquez-Aguirre, Hernández-Gutiérrez Rodolfo, Pablo César Ortiz-Lazareno, Gabriela Chavez-Calvillo, Mauricio Carrillo-Tripp, Laura Silva-Rosales3 and Abel Gutiérrez-Ortega. Virology Journal. 2012, 9 (75) pp 1-11. March 27, 2012. DOI:10.1186/1743-422X-9-75.

Trafficking of immature F508-CFTR to the plasma membrane and its detection by biotinylation. Yishan Luo, Ken McDonald and John W. Hanrahan. Biochem Journal. 2009, 419 pp 211–219. December 8, 2008. DOI:10.1042/BJ20081869.

Generation of streptavidin-tagged human-granulocyte macrophage colony-stimulating factor fusion proteins. Bai Li, Hu Zhiming, Wang Fei, Xu Xiaoling, Xia Chang, Jin Liqin, Li Jinlong, Gao Jimin. Pub Med. 2012, 32 (10) pp 1389-1393. October 1, 2012. DOI: PMID:23076170.

Mitoxantrone Targets the ATP-binding Site of FAK, Binds the FAK Kinase Domain and Decreases FAK, Pyk-2, c-Src, and IGF-1R, In Vitro Kinase Activities. Vita Golubovskaya, Baotran Ho, Min Zheng, Andrew Magis, David Ostrov and William Cance. Anticancer Agents Med Chem. 2013, 13 (4) pp 546-554. May 1, 2013. DOI: PMC3625494.

Single-Molecule Detection for Femtomolar Quantification of Proteins in Heterogeneous Immunoassays. Eric A. Nalefski, Christina M. D’Antoni, Evan P. Ferrell, Janice A. Lloyd, Haoqun Qiu, John L. Harris, and Duncan H. Whitney. Clinical Chemistry. 2006, 52 (11) pp 2172-2175. November 1, 2006. DOI: 10.1373/clinchem.2006.072850.

Micro-total analysis system for virus detection: microfluidic pre-concentration coupled to liposome-based detection. John T. Connelly, Sowmya Kondapalli, Marc Skoupi, John S. Parker, Brian J. Kirby, Antje J. Baeumner. Analytical and Bioanalytical Chemistry. 2012, 402 (1) pp 315-323. September 10, 2011. DOI: 10.1007/s00216-011-5381-9.

Formation of raloxifene homo-dimer in CYP3A4, evidence for multi-substrate binding in a single catalytically competent P450 active site. John A. Davis, Robert J. Greene, Sean Han, Dan A. Rock, Larry C. Wienkers. Archives of Biochemistry and Biophysics. 2011, 513 (2) pp 110-118. September 15, 2011. doi:10.1016/

Comparative study of thiolated protein G scaffolds and signal antibody conjugates in the development of electrochemical immunosensors. Jeremy M. Fowler, Margaret C. Stuart, Danny K.Y. Wong. Europe PubMed Central. 2007, 23 (5) pp 633-639. July 28, 2007. DOI: 10.1016/j.bios.2007.07.007.

Detection of viral bioagents using a shear horizontal surface acoustic wave biosensor. M. Bisoffi, B. Hjelle, D.C. Brown, D.W. Branch, T.L. Edwards, S.M. Brozik, V.S. Bondu-Hawkins, R.S. Larson. Biosensors and Bioelectronics. 2008, 23 (9) pp 1397-1403. April 15, 2008. DOI: 10.1016/j.bios.2007.12.016.

Reduction of matrix interferences by the combination of chaotropic salt and DMSO in a broadly applicable target-based ELISA for pharmacokinetic studies of therapeutic monoclonal antibodies. Julie Doucet , Jasna Canadi, Christoph Kalis, Marie-Anne Valentin, Séverine Marrony ,Fabienne Deckert-Salva, Francois Legay, Alexandre Avrameas. Journal of Pharmaceutical and Biomedical Analysis. 2009, 50 (5) pp 924-931. December 5, 2009. DOI :10.1016/j.jpba.2009.06.029.

Plasma Profiling Reveals Human Fibulin-1 as Candidate Marker for Renal Impairment. Maja Neiman, Jesper J. Hedberg, Pierre R. D€onnes, Ina Schuppe-Koistinen, Stephan Hanschke, Ralf Schindler, Mathias Uhl’en, Jochen M. Schwenk, and Peter Nilsson. Journal of Proteome Research. 2011, 10 (11) pp 4925-4934. September 3, 2011.

DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device. Ying-Ja Chen, Eric E. Roller and Xiaohua Huang. Lab Chip. 2010, 10 (9) pp 1153-1159. May 7, 2010. DOI: 10.1039/b921417h.

Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow. Sanford H. Leuba, Travis B. Wheeler , Chao-Min Cheng , Philip R. LeDuc , Mónica Fernández-Sierra , Edwin Quiñones. Methods. 2009, 47 (3) pp 214-222. November 17, 2008. DOI:10.1016/j.ymeth.2008.10.022.

Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis. Kerstin Ho¨ner, zu Bentrup,Rajee Ramamurthy,C. Mark Ott , Kamal Emami, Mayra Nelman-Gonzalez , James W. Wilson , Emily G. Richter , Thomas J. Goodwin , J. Stephen Alexander , Duane L. Pierson , Neal Pellis , Kent L. Buchanan , Cheryl A. Nickerson. Microbes and Infection. 2006, 8 (7) pp 1813-1825. April 27, 2006. DOI:10.1016/j.micinf.2006.02.020.

Engineering the binding properties of the T cell receptor:peptide:MHC ternary complex that governs T cell activity. Natalie A. Bowerman, Terence S. Crofts, Lukasz Chlewicki, Priscilla Do, Brian M. Baker, K. Christopher Garcia, David M. Kranz. Molecular Immunology. 2009, 46 (15) pp 3000-3008. June 12, 2009. DOI: 10.1016/j.molimm.2009.06.012.

High-Throughput Screening of Small Molecules Identifies Hepcidin Antagonists. Eileen Fung, Priscilla Sugianto, Jason Hsu, Robert Damoiseaux, Tomas Ganz, and Elizabeta Nemeth. Molecular Pharmacology. 2013, 83 (3) pp 681-690. January 4, 2013. DOI: org/10.1124/mol.112.083428.

Identification of Quantum Dot Bioconjugates and Cellular Protein Co-localization by Hybrid Gel Blotting. Hong Yan Liu and Tania Q. Vu. Nano Letters. 2007, 7 (4) pp 1044-1049. March 3, 2007. DOI: 10.1021/nl070239e.

Multilayer enzyme-coupled magnetic nanoparticles as efficient, reusable biocatalysts and biosensors. Josep Garcia, Yue Zhang, Hannah Taylor, Oscar Cespedes, Michael E. Webb and Dejian Zhou. Nanoscale. 2011, 3 (9) pp 3721-3730. June 10, 2011. DOI: 10.1039/C1NR10411J.

Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein. Yaji Tian, Matthew J. Cuneo, Anita Changela, Birte Hocker, Lorena S. Beese, and Homme W. Hellinga. Protein Science. 2007, 16 (10) pp 2240-2250. January 1, 2009. DOI: 10.1110/ps.072969407.

Generation of monospecific antibodies based on affinity capture of polyclonal antibodies. Barbara Hjelm, Bjorn Forsstrom, Ulrika Igel, Henrik Johannesson, Charlotte Stadler, Emma Lundberg, Fredrik Ponten, Anna Sjoberg, Johan Rockberg, Jochen M. Schwenk, Peter Nilsson, Christine Johansson, and Mathias Uhlen. Protein Science. 2011, 20 (11) pp 1824-1835. October 12, 2011. DOI: 10.1002/pro.716.

Antibody-based profiling of cerebrospinal fluid within multiple sclerosis. Anna Haggmark, Sanna Bystrom, Burcu Ayoglu, Ulrika Qundos, Mathias Uhlen, Mohsen Khademi, Tomas Olsson, Jochen M. Schwenk and Peter Nilsson. Proteomics, 2013, 13 (15) pp 2256-2267. June 25, 2013. DOI: 10.1002/pmic.201200580.

Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display. Kip Dudgeon , Romain Rouet , Kristoffer Famm , and Daniel Christ. Methods in Molecular Biology. 2012, 911 pp 383-397. July 12, 2012. DOI: 10.1007/978-1-61779-968-6_23.

Highly sensitive Mach–Zehnder interferometer biosensor based on silicon nitride slot waveguide. Qing Liu, Xiaoguang Tu, Kyung Woo Kim, Jack Sheng Kee, Yong Shin, Kyungsup Han, Yong-Jin Yoon, Guo-Qiang Lo, Mi Kyoung Park. Sensors and Actuators B: Chemical. 2013, 188 pp 681-688. July 25, 2013. DOI: 10.1016/j.snb.2013.07.053.

Neuregulin-1 is neuroprotective in a rat model of organophosphate-induced delayed neuronal injury. Yonggang Li , Pamela J. Lein , Cuimei Liu , Donald A. Bruun , Cecilia Giulivi , Gregory D. Ford , Teclemichael Tewolde , Catherine Ross-Inta , Byron D. Ford. Toxicology and Applied Pharmacology. 2012, 262 (2) pp 194-204. July 15, 2012. DOI: 10.1016/j.taap.2012.05.001.

Detection of human platelet antigen-1a alloantibodies in cases of fetomaternal alloimmune thrombocytopenia using recombinant b3 integrin fragments coupled to fluorescently labeled beads. Winnie Chong, Paul Metcalfe, Rosey Mushens, Geoff Lucas,Willem H. Ouwehand, and Cristina V. Navarrete. Transfusion. 2010, 51 (6) pp 1261-1270. December 16, 2010. DOI: 10.1111/j.1537-2995.2010.02977.x.

A plant derived multifunctional tool for nanobiotechnology based on Tomato bushy stunt virus. Simone Grasso, Chiara Lico, Francesca Imperatori, Luca Santi. Transgenic Research. 2013, 22 (3) pp 519-535. October 30, 2012. DOI:10.1007/s11248-012-9663-6.

An analysis of the function and expression of D6 on lymphatic endothelial cells. Clive S. McKimmie, Mark D. Singh, Kay Hewit, Oscar Lopez-Franco, Michelle Le Brocq, Stefan Rose-John, Kit Ming Lee, Andrew H. Baker, Rachel Wheat, David J. Blackbourn, Robert J. B. Nibbs and Gerard J. Graham. Blood Journal. 2013, 121 pp 3768-3777. March 11, 2013. DOI:10.1182/blood-2012-04-425314.

Light-Up Hoechst–DNA Aptamer Pair: Generation of an Aptamer-Selective Fluorophore from a Conventional DNA-Staining Dye. Shinsuke Sando, Atsushi Narita, and Yasuhiro Aoyama. ChemBioChem. 2007, 8 (15) pp 1795-1803. October 15, 2007. DOI: 10.1002/cbic.200700325.

A Fresnel zone plate biosensor for signal amplification with enhanced signal-to-noise ratiow. Yong-Cheol Jeong, Bokyung Jung, Jung-Hwan Park and Jung-Ki Park. Chemical Communications. 2012, 48 (51) pp 6378-6380. May 2, 2012. DOI: 10.1039/C2CC32008H.

Anti-tumor efficacy of chitosan-g-poly(ethylene glycol) nanocapsules containing docetaxel: Anti-TMEFF-2 functionalized nanocapsules vs. non-functionalized nanocapsules. Daniel Torrecilla, Maria V. Lozano, Enrique Lallana, Jose I. Neissa, Ramon Novoa-Carballal, Anxo Vidal, Eduardo Fernandez-Megia, Dolores Torres, Ricardo Riguera, Maria J. Alonso, Fernando Dominguez. European Journal of Pharmaceutics and Biopharmaceutics. 2013, 83 (3) pp 330-337. April 1, 2013. DOI: 10.1016/j.ejpb.2012.10.017.

Robust dithiocarbamate-anchored amine functionalization of Au nanoparticles. Kai Chen, Hans D. Robinson. Journal of Nanoparticle Research. 2011, 13 (2) pp 751-761. February 1, 2011. DOI: 10.1007/s11051-010-0075-3.

Autocatalytic Activation of Influenza Hemagglutinin. Jeong H. Lee, Mark Goulian, Eric T. Boder. Journal of Molecular Biology. 2006, 364 (3) pp 275-282. December 1, 2006. DOI: 10.1016/j.jmb.2006.09.015.

All major prion types recognised by a multiplex immunofluorometric assay for disease screening and confirmation in sheep. Yue Tang, Adriana Gielbert, Jorg G. Jacobs, Thierry Baron, Olivier Andreoletti, Takashi Yokoyama, Jan P.M. Langeveld, Maurice J. Sauer. Journal of Immunological Methods. 2012, 380 (1-2) pp 30-39. June 29, 2012. DOI: 10.1016/j.jim.2012.03.004.

An extended range generic immunoassay for total human therapeutic antibodies in preclinical pharmacokinetic studies. Colin M. Hall, Josh T. Pearson, Vimal Patel, Larry C. Wienkers, Robert J. Greene. Journal of Immunological Methods. 2013, 393 (1-2) pp 70-73. July 31, 2013. DOI: 10.1016/j.jim.2013.03.011.

Characterization and use of a rabbit-anti-mouse VPAC1 antibody by flow cytometry. Rebecca J. Hermann, Travis Van der Steen, Emilie E. Vomhof-DeKrey, Sejaa Al-Badrani, Steve B. Wanjara, Jarrett J. Failing, Jodie S. Haring, Glenn P. Dorsam. Journal of Immunological Methods. 2012, 376 (1-2) pp 20-31. February 28, 2012. DOI:10.1016/j.jim.2011.10.009.

Possible involvement of glycolipids in lectin-mediated cellular transformation of symbiotic microalgae in corals. Mitsuru Jimbo, Yuya Suda, Kazuhiko Koike, Sachiko Nakamura-Tsuruta, Junko Kominami, Masugu Kamei, Jun Hirabayashi Ryuichi Sakai, Hisao Kamiya. Journal of Experimental Marine Biology and Ecology. 2013, 439 pp 129-135. January 1, 2013. DOI: 10.1016/j.jembe.2012.10.022.

Non-healing is associated with persistent stimulation of the innate immune response in chronic venous leg ulcers. Brita S. Pukstad, Liv Ryan, Trude H. Flo, Jørgen Stenvik, Ryan Moseley, Keith Harding, David W. Thomas, Terje Espevik. Journal of Dermatological Science. 2010, 59 (2) pp 115-122. August 1, 2010. DOI: 10.1016/j.jdermsci.2010.05.003.

G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker. Xiaoyi Zheng, Frank Chang, Xinmin Zhang, Vicki L. Rothman, George P. Tuszynski. Experimental and Molecular Pathology. 2012, 93 (1) pp 111-115. August 1, 2012. DOI: 10.1016/j.yexmp.2012.03.013.

P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity. Anja W. Fjorback, Sabrina Sundbye, Justus C. Dachsel, Steffen Sinning, Ove Wiborg and Poul H. Jensen. FEBS Journal. 2011, 278 (3) pp 493-505. December 23, 2010. DOI: 10.1111/j.1742-4658.2010.07970.x.

Quantitative analysis of multivesicular bodies (MVBs) in the hypoglossal nerve: Evidence that neurotrophic factors do not use MVBs for retrograde axonal transport. Amy L. Altick, Larisa M. Baryshnikova, Tania Q. Vu and Christopher S. von Bartheld. Journal of Comparative Neurology. 2009, 514 (6) pp 641-657. March 18, 2009. DOI: 10.1002/cne.22047.

Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. Kazuhide Watanabe, Tadahiro Nagaoka, Joseph M. Lee, Caterina Bianco, Monica Gonzales, Nadia P. Castro, Maria Cristina Rangel, Kei Sakamoto, Youping Sun, Robert Callahan, and David S. Salomon. JCB. 2009, 187 (3) pp 343–353. November 2, 2009. DOI: 10.1083/jcb.200905105.

Identification of the human eosinophil lineage-committed progenitor: revision of phenotypic defi nition of the human common myeloid progenitor. Yasuo Mori, Hiromi Iwasaki, Kentaro Kohno, Goichi Yoshimoto, Yoshikane Kikushige, Aki Okeda, Naokuni Uike, Hiroaki Niiro, Katsuto Takenaka, Koji Nagafuji, Toshihiro Miyamoto, Mine Harada, Kiyoshi Takatsu, and Koichi Akashi. The Journal of Experimental Medicine. 2008, 206 (1) pp 183-193. December 29, 2008. DOI: 10.1084/jem.20081756.

AL Amyloid Imaging and Therapy with a Monoclonal Antibody to a Cryptic Epitope on Amyloid Fibrils. Jonathan S. Wall, Stephen J. Kennel, Angela Williams, Tina Richey, Alan Stuckey, Ying Huang, Sallie Macy, Robert Donnell, Robin Barbour, Peter Seubert, Dale Schenk. PLOS ONE. 2012, 7 (12) pp e52686. December 26, 2012. DOI: 10.1371/journal.pone.0052686.

Multidimensional nano-HPLC coupled with tandem mass spectrometry for analyzing biotinylated proteins. Jens Sproß, Sebastian Brauch, Friedrich Mandel, Moritz Wagner, Stephan Buckenmaier, Bernhard Westermann, Andrea Sinz. Analytical and Bioanalytical Chemistry. 2012, 405 (7) pp 2163-2173. May 29, 2012. DOI: 10.1007/s00216-012-6057-9.

Generation and characterization of Anti-AA Amyloid-Specific Monoclonal Antibodies. Jonathan S. Wall , Stephen J. Kennel, Tina Richey, Amy Allen, Alan Stuckey, Deborah T. Weiss, Sallie D. Macy, Robin Barbour, Peter Seubert , Alan Solomon and Dale Schenk. Frontiers in Immunology. 2011, 2 (32). August 8, 2011. DOI: 10.3389/fimmu.2011.00032.

a-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling. Myriam M. Ouberai, Juan Wang, Marcus J. Swann, Celine Galvagnion, Tim Guilliams, Christopher M. Dobson and Mark E. Welland. The Journal of Biological Chemistry. 2013, June 5, 2013. DOI: 10.1074/jbc.M113.478297.

Site-Specific Labeling of DNA and RNA Using an Efficiently Replicated and Transcribed Class of Unnatural Base Pairs. Young Jun Seo, Denis A. Malyshev, Thomas Lavergne, Phillip Ordoukhanian, and Floyd E. Romesberg . Journal of the American Chemical Society. 2011, 133 (49) pp 19878–19888. October 8, 2011. DOI: 10.1021/ja207907d.

Antibody Repertoire Profiling Using Bacterial Display Identifies Reactivity Signatures of Celiac Disease. Bradley N. Spatola, Joseph A. Murray, Martin Kagnoff, Katri Kaukinen, and Patrick S. Daugherty. Analytical Chemistry. 2013, 85 (2) pp 1215–1222. December 12, 2012. DOI: 10.1021/ac303201d.

Design of a prototype flow microreactor for synthetic biology in vitro. Christian R. Boehm, Paul S. Freemont and Oscar Ces. Lab on a Chip. 2013, 13 (17) pp 3426-3432. June 27, 2013. DOI: 10.1039/c3lc50231g.

A visualized observation of calcium-dependent gelsolin activity upon the surface coverage of fluorescent-tagged actin filaments. Yongkuk Lee, Ming-Yuan Wei, Parviz Famouri. Journal of Colloid and Interface Science. 2013, 389 (1) pp 182–187. January 1, 2013. DOI: 10.1016/j.jcis.2012.08.049.

Selective attachment of F-actin with controlled length for developing an intelligent nanodevice. Ming-Yuan Wei, Lenin J. Leon, Yongkuk Lee, Denzel Parks, Lloyd Carroll, Parviz Famouri. Journal of Colloid and Interface Science. 2011, 356 (1) pp 182-189. April 1, 2011. DOI: 10.1016/j.jcis.2010.12.079.

A pretargeted nanoparticle system for tumor cell labeling. Jonathan Gunn, Steven I. Park, Omid Veiseh, Oliver W. Press and Miqin Zhang. Molecular BioSystems. 2011, 7 (3) pp 742-748. October 13, 2010. DOI: 10.1039/c005154c.

Directional Transport by Nonprocessive Motor Proteins on Fascin-Cross-Linked Actin Arrays. Yongkuk Lee and Parviz Famouri. Nano Letter. 2013, 13 (8) pp 3775–3782. July 2, 2013. DOI: 10.1021/nl401718q.

Layer-by-Layer Assembly of Bioengineered Flagella Protein Nanotubes. Mudalige Thilak Kumara, Brian C Tripp, Subra Muralidharan. Biomacromolecules. 2007, 8 (12) pp 3718-3722. November 03, 2007. DOI: 10.1021/bm7005449.

Preparation of Biotinylated Cypridina Luciferase and Its Use in Bioluminescent Enzyme Immunoassay. Chun Wu, Kosei Kawasaki, Yoko Ogawa, Yasukazu Yoshida, Satoru Ohgiya, and Yoshiro Ohmiya. Analytical Chemistry. 2007, 79 (4) pp 1634-1638. January 13, 2007. DOI: 10.1021/ac061754k.

Prion Protein Detection Using Nanomechanical Resonator Arrays and Secondary Mass Labeling. Madhukar Varshney , Philip S. Waggoner , Christine P. Tan , Keith Aubin , Richard A. Montagna , and Harold G. Craighead. Analytical Chemistry . 2008, 80 (6), pp 2141–2148. February 14, 2008. DOI: 10.1021/ac702153p

Enhancing Peptide Ligand Binding to Vascular Endothelial Growth Factor by Covalent Bond Formation. Bernadette V. Marquez, Heather E. Beck,Tolulope A. Aweda, Brett Phinney, Cynthia Holsclaw, William Jewell, Diana Tran, Jeffrey J. Day, Malalage N. Peiris, Charles Nwosu,Carlito Lebrilla, and Claude F. Meares.Bioconjugate Chemistry. 2012, 23 (5) pp 1080-1089. April 26, 2012. DOI: 10.1021/bc300114d.

Introduction of the Mass Spread Function for Characterization of Protein Conjugates. Joseph P. Skinner, Lianli Chi, Panfilo F. Ozeata,Carol S. Ramsay, Robynn L. O’Hara, Brenda B. Calfin, and Sergey Y. Tetin. Analytical Chemistry. 2012, 84 (2), pp 1172–1177. November 30, 2011. DOI: 10.1021/ac202239j

Electrochemical Template Synthesis of Multisegment Nanowires:  Fabrication and Protein Functionalization. Bridget Wildt, Prashant Mali, and Peter C. Searson. Langmuir. 2006, 22 (25), pp 10528–10534. September 8, 2006. DOI: 10.1021/la061184j.

Synthetic, site-specific biotinylated analogs of human MCP-1. Marian Kruszynski, Ping Tsui, Nicole Stowell, Jinquan Luo, Jennifer F. Nemeth, Anuk M. Das, Raymond Sweet, George A. Heavner. Journal of Peptide Science. 2006, 12 (5) pp 354-360. November 14, 2005. DOI: 10.1002/psc.734.

Biotinylation reagents for the study of cell surface proteins. Giuliano Elia. Proteomics. 2008, 8 (19) pp 4012-4024. September 3, 2008. DOI: 10.1002/pmic.200800097.

Melanoma Imaging with Pretargeted Bivalent Bacteriophage. Jessica R Newton, Yubin Miao, Susan L Deutscher, Thomas P Quinn. Journal of Nuclear Medicine. 2007, 48 (3) pp 429-436. March 1, 2007.

Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm. Suci P, Kang S, Gmür R, Douglas T, Young M.. Antimicrobial Agents and Chemotherapy. 2010, 54 (6) pp 2489-2496. April 12, 2010. DOI: 10.1128/AAC.00059-10.

The therapeutic potential of SA-sCD40L in the orthotopic model of superficial bladder cancer. Zhang Z, Xu X, Zhang X, Chen X, Chen Q, Dong L, Hu Z, Li J, Gao J.Acta Oncologica. 2011, 50 (7) pp 1111-1118. October 1, 2011. DOI: 10.3109/0284186X.2010.549838.

Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform. Nicole C. Hauser, Rafael Martinez, Anette Jacob, Steffen Rupp, Jörg D. Hoheisel, and Stefan Matysiak. Nucleic Acids Research. 2006, 34 (18) pp 5101–5111. September 20, 2006. DOI: 10.1093/nar/gkl671.

The Lysosomal Transmembrane Protein 9B Regulates the Activity of Inflammatory Signaling Pathways. Francis Dodeller, Marie Gottar, Dieter Huesken, Vadim Iourgenko and Bruno Cenni. The Journal of Biological Chemistry. 2008, 283 (31) pp 21487-21494. June 9, 2008. DOI: 10.1074/jbc.M801908200.

The Nutrigenetics of Hyperhomocysteinemia. Patricia M. DiBello, Sanjana Dayal, Suma Kaveti, Dongmei Zhang, Michael Kinter, Steven R. Lentz, and Donald W. Jacobsen. Molecular & Cellular Proteomics. 2010, 9 (3) pp 471-485. December 21, 2009. 10.1074/mcp.M900406-MCP200.

Targeted Contrast-Enhanced Ultrasound Imaging of Tumor Angiogenesis with Contrast Microbubbles Conjugated to Integrin-Binding Knottin Peptides. Jürgen K. Willmann, Richard H. Kimura, Nirupama Deshpande, Amelie M. Lutz, Jennifer R. Cochran and Sanjiv S. Gambhir. The Journal of Nuclear Medicine. 2010, 51 (3) pp 433-440. February 11, 2010. DOI: 10.2967/jnumed.109.068007.

Sequence determinants of protein aggregation in human VH domains. Kip Dudgeon, Kristoffer Famm, and Daniel Christ. Protein Engineering, Design & Selection. 2009, 22 (3) pp 217-220. October 28, 2008. DOI: 10.1093/protein/gzn059.

Rapid Tumor Necrosis Factor α-Induced Exocytosis of Glutamate Receptor 2-Lacking AMPA Receptors to Extrasynaptic Plasma Membrane Potentiates Excitotoxicity. Dmitri Leonoudakis, Pingwei Zhao, and Eric C. Beattie. The Journal of Neuroscience. 2008, 28 (9) pp 2119-2130. February 27, 2008. DOI: 10.1523/JNEUROSCI.5159-07.2008.

Polymorphisms of Mouse Apolipoprotein A-II Alter Its Physical and Functional Nature. Timothy J. Sontag, Catherine A. Reardon. PLOS ONE. 2014, 9 (2) e88705. February 10, 2014. DOI:10.1371/journal.pone.0088705.

DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells. Yinghao Zhang, Fang-Mei Chang, Jianjun Huang, Jacob J. Junco, Shivani K. Maffi, Hannah I. Pridgen, Gabriel Catano, Hong Dang, Xiang Ding, Fuquan Yang, Dae Joon Kim, Thomas J. Slaga, Rongqiao He, Sung-Jen Wei. Protein & Cell. 2013, 5 (2) pp 124-140. February 11, 2014. DOI: 10.1007/s13238-013-0018-8.

Structurally Defined Nanoscale Sheets from Self-Assembly of Collagen-Mimetic Peptides. Tao Jiang, Chunfu Xu, Yang Liu, Zheng Liu, Joseph S. Wall, Xiaobing Zuo, Tianquan Lian, Khalid Salaita, Chaoying Ni, Darrin Pochan, Vincent P. Conticello. Journal of the American Chemical Society. 2014, 136 (11) pp 4300-4308. February 26, 2014. DOI: 10.1021/ja412867z.

Linker length matters, Fynomer – Fc Fusion with an Optimized Linker Displaying Picomolar IL-17A Inhibition Potency. Michela Silacci, Nadja Baenziger-Tobler, Wibke Lembke, Wenjuan Zha, Sarah Batey, Julian Bertschinger and Dragan Grabulovski. The Journal of Biological Chemistry. 2014, 289 (14) pp 14392-14398. April 1, 2014. DOI: 10.1074/jbc.M113.534578.

Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge. Gang Chen, Jayne F. Koellhoffer, Samantha E. Zak, Julia C. Frei, Nina Liu, Hua Long, Wei Ye, Kaajal Nagar, Guohua Pan, Kartik Chandran, John M. Dye, Sachdev S. Sidhu, and Jonathan R. Lai. ACS Chemical Biology. 2014, 9 (10) pp 2263-2273. August 20, 2014. DOI: 10.1021/cb5006454.

New Assay for Quantification of PEGylated Proteins During in Vitro Permeability Studies. Spela Jalen,, Vanja Smilovic, Katarina Fidler, Barbara Podobnik, Maja Marusic, Radovan Komel, Vladka Gaberc-Porekar and Simona Jevsevar. Acta Chimica Slovenica. 2014, 61 (3) pp 615-622. January 15, 2014.

Calsyntenin3: Molecular Architecture and Interaction with Neurexin 1alpha. Zhuoyang Lu, Yun Wang, Fang Chen, Huimin, Tong, M. V.V.V. Sekhar Reddy, Lin Luo, Suchithra Seshadrinathan, Lei Zhang, Luis, Marcelo F. Holthauzen, Ann Marie Craig, Gang Ren and Gabby Rudenko. Journal of Biological Chemistry. 2014, 289 (50) pp 34530-34542. October 28, 2014. DOI: 10.1074/jbc.M114.606806.

Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli. Jaakko M. Itkonen, Arto Urtti, Louise E. Bird, Sanjay Sarkhel. BMC Biotechnology. 2014, 14 (92). November 14, 2014. DOI: 10.1186/s12896-014-0092-x.

Analysis of the sensitizing properties of ragweed pollen componets and the influence of environmental factors in a murine in vivo model. Maria Kamml. Fakultat fur Medizin. 2014, July 5, 2014.

Adapter Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine a-Amidating Monooxygenase, a Luminal Cuproenzyme. Mathilde L. Bonnemaison, Nils Back, Megan E. Duffy, Martina Ralle, Richard E. Mains and Betty A. Eipper. Journal of Biological Chemistry. 2015. July 13, 2015. DOI: 10.1074/jbc.M115.641027.

Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. Janek Kibat, Thomas Schirrmann, Matthias J. Knape, Saskia Helmsing, Doris Meier, Michael Hust, Christoph Schroder, Daniela Bertinetti, Gerhard Winter, Khalid Pardes, Mia Funk, Andrea Vala, Nathalia Giese, Friedrich W. Herberg, Stefan Dubel, and Jorg D. Hoheisel. New Biotechnology. 2015, 00 (00). December 17, 2015. DOI: 101016/j.nbt.2015.12.003.

Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations. Christian Margreitter, Patrick Mayrhofer, Renate Kunert, and Chris Oostenbrink. Journal of Molecular Recognition. 2016, January 8, 2016. DOI: 10.1002/jmr.2527.

The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases. Carmen Bergom, Andrew D. Hauser, Amy Rymaszewski, Patrick Gonyo, Jeremy W. Prokop, Benjamin C. Jennings, Alexis J. Lawton, Anne Frei, Ellen L. Lorimer Jr., Irene Aguilera-Barrantes Jr., Alexander C. Mackinnon Jr., Kathleen Noon, Carol A. Fierke, and Carol L. Williams. The Journal of Biological Chemistry. 2016, January 26, 2016. DOI: 10.1074/jbc.M115.696831.

Achieving Potent Autologous Neutralizing Antibody Responses against Tier 2 HIV-1 Viruses by Strategic Selection of Envelope Immunogens. Ann J. Hessell, Delphine C. Malherbe, Franco Pissani, Sean McBurney, Shelly J. Krebs, Michelle Gomes, Shilpi Pandey, William F. Sutton, Benjamin J. Burwitz, Matthew Gray, Harlan Robins, Byung S. Park, Jonah B. Sacha, Celia C. LaBranche, Deborah H. Fuller, David C. Montefiori, Leonidas Stamatatos, D. Noah Sather, and Nancy L. Haigwood. The Journal of Immunology. 2016, pp 1550-6606. March 4, 2016. DOI: 10.4049/jimmunol.1500527.

Nanometric gap structure with fluid lipid bilayer for the selective transport and detection of biological molecules. Koji Ando, Masashi Tanabe, and Kenichi Morigaki. Langmuir. 2016, pp 1-30. July 18, 2016. DOI: 10.1021/acs.langmuir.6b01405.

Tagging and Enriching Proteins Enables Cell-Specific Proteomics. Thomas S. Elliott, Ambra Bianco, Fiona M. Townsley, Stephen D. Fried, and Jason W. Chin. Cell Chemical Biology. 2016, 23 pp 805-815. July 21, 2016.

Thrombospondin-1 Mimetic Agonist Peptides Induce Selective Death in Tumor Cells: Design, Synthesis and Structure-Activity Relationship Studies. Thomas Denefle, Heloise Boullet, Linda Herbi, Clara Newton, Ana-Carolina, Martinez-Torres, Alexandre Guez, Elodie Pramil, Claire Quiney, Marilyne Pourcelot, Mikail Daniel Levasseur, Eva Larde, Roba Moumne, Francois-Xavier Ogi, Pascal Grondin, Helene Merle-Beral, Olivier Lequin, Santos A. Susin, and Philippe Karoyan. Journal of Medicinal Chemistry. 2016, pp 1-33. August 15, 2016. DOI: 10.1021/acs.jmedchem.6b00781.

Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents. Barbara Bernardim, Pedro M.S.D. Cal, Maria J. Matos, Bruno L. Oliveira, Nuria Martinez-Saez, Ines S. Albuquerque, Elizabeth Perkins, Francisco Corzana, Antonio C.B. Burtoloso, Gonzalo Jimenez-Oses, and Goncalo J.L. Bernardes. Nature Communications. 2016, 7 (13128) pp 1-9. October 26, 2016. DOI: 10.1038/ncomms13128.

Surface Plasmon Resonance Imaging Microscopy of Liposomes and Liposome-Encapsulated Gold Nanoparticles. Lauri Viitala, Adam M Maley, H.W. Millie Fung, Robert M. Corn, Tapani Viitala, and Lasse Murtomaki. The Journal of Physical Chemistry. 2016, October 31, 2016. DOI: 10.1021/acs.jpcc.6b09503.

A digital enzyme-linked immunosorbent assay for ultrasensitive measurement of amyloid-ß 1-42 peptide in human plasma with utility for studies of Alzheimer’s disease therapeutics. Linan Song, D Richard Lachno, David Hanlon, Adam Shepro, Andreas Jeromin, Dipika Gemani, Jayne A Talbot, Margaret M Racke, Jeffrey L Dage, and Robert A Dean. Alzheimer’s Research & Therapy. 2016, 8 (58) pp 1-15. December 15, 2016. DOI: 10.1186/s13195-016-0225-7.

A versatile approach towards nucleobase-modified aptamers. Fabian Tolle, Dr. Gerhard M Brandle, Daniel Matzner, and Prof. Dr. Gunter Mayer. Angewandte Chemie. 2015, 54 (37) pp 10971-10974. September 7, 2015. DOI: 10.1002/anie.201503652.

Transport of biomolecules to binding partners displayed on the surface of microbeads arrayed in traps in a microfluidic cell. Xiaoxiao Chen, Thomas F Leary, and Charles Maldarelli. Biomicrofluidics. 2017, 11 (1) pp 014101. January 4, 2017. DOI: 10.1063/1.4973247.

Affinity purification of human m-calpain through an intrinsically disordered inhibitor, calpastatin. Hung Huy Nguyen, Mihaly Varadi, Peter Tompa, and Kris Pauwels. PLOS one. 2017, 12 (3): e0174125. March 20, 2017.

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