Alkyne Sphinganine (CCT-1452)

Description

Alkyne Sphinganine a metabolic labeling reagent that contains a terminal alkyne moiety that allows for the subsequent tagging of protein−lipid complexes by click chemistry. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst allowing for the visualization and proteomic profiling of protein−sphingolipid complexes in living cells.

Specifications

Documents

Selected References

1. Gaebler A., et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid Res., 57, 1934-47. [PubMed]
2. Gaebler A., et al. (2013). Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays. J. Lipid Res., 54, 2282-90. [PubMed]
3. Lee, M.T., et al. (2020). Dietary sphinganine is selectively assimilated by members of themammaliangut microbiome. J Lipid Res . [PubMed]