Vector Labs is closed to observe Independence Day (US Holiday) on Thursday, July 4th.
We will be back in the office on Friday. We will respond to emails upon our return. Have a wonderful day.

Agarose-GLAGLLAR-Alkyne (CCT-1582)

[text-backorder]

Description

Agarose-GLAGLLAR-Alkyne is an agarose resin that is pre-activated with terminal alkyne groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The azide-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Alkyne resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, azide-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 113.05 Da

Specifications

Unit Size2 ml
Enrichment target
Azide-modified proteins
Format
Batch or column purifications
Activation level
5-12 µmol alkyne groups per mL resin
Bead sizeSpherical, 50-150 µm
Support6% Cross-linked agarose
Formulation50% slurry
Preservative20% ethanol
AppearanceOff-white slurry
Storage2-8C
Shipping ConditionsAmbient temperature

Selected References

  1. Shao, Y., et al. (2021). Enhancing Comprehensive Analysis of Newly Synthesized Proteins Based on Cleavable Bioorthogonal Tagging. Anal Chem.93 (27), 9408-9417. [PubMed]

Applicable patents and legal notices are available at legal notices.

Related Products

How do I Request a Quote?

To request a quote for products:

  1.  Sign in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote