Agarose-GLAGLLAR-Alkyne (CCT-1582)

Description

Agarose-GLAGLLAR-Alkyne is an agarose resin that is pre-activated with terminal alkyne groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The azide-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Alkyne resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, azide-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 113.05 Da

Specifications

Documents

Selected References

1. Shao, Y., et al. (2021). Enhancing Comprehensive Analysis of Newly Synthesized Proteins Based on Cleavable Bioorthogonal Tagging. Anal Chem., 93 (27), 9408-9417. [PubMed]