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Inhibiting Sugars and Eluting Reagents

Glycoproteins are frequently isolated and purified from protein mixtures using columns of agarose-bound lectins.  After applying a protein mixture, the agarose-lectin column is washed free of unwanted proteins and the glycoprotein bound to the lectin is eluted with a sugar that inhibits binding.  Unfortunately, achieving complete elution with a simple sugar solution can be difficult.  The Glycoprotein Eluting Solutions, in the neutral pH range, maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use.  All components of these ready-to-use Glycoprotein Eluting Solutions can be subsequently removed by dialysis.

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