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PHOTOPROBE® Biotin Nucleic Acid Labeling and Detection System

Catalog Number: SPK-1906

Availability: In stock

$365.00
Unit Size: 1 kit

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Overview

    PHOTOPROBE® Biotin Labeling and Detection System provides the essential reagents for labeling DNA or RNA probes with biotin and detecting these probes on nitrocellulose and nylon membranes, or tissue sections with a chromogenic substrate.

    Components of the PHOTOPROBE® Biotin Labeling and Detection System:

    • PHOTOPROBE® (Long Arm) Biotin Labeling Kit (labels up to 250 μg of target)
    • 10x Casein Solution (250 ml concentrate)
    • Alkaline Phosphatase Streptavidin (1 ml)
    • BCIP/NBT Substrate Kit

    Features of PHOTOPROBE® Biotin Labeling Kit:

    • A method of choice for incorporating biotin at multiple sites along the entire length of the nucleic acid. This method does not destroy the original nucleic acid nor create its copy.
    • Labels DNA, RNA or PNA. 
    • Kit labels up to 250 mg of target or up to 50 labeling reactions. 

    Contents of the PHOTOPROBE® Biotin Labeling Kit:

    • PHOTOPROBE® Biotin Reagent
    • Tris Buffer
    • sec-Butanol
    • Precipitant
    • Biotinylated DNA Standard

     

     

    Additional Information

    Catalog Number SPK-1906
    Unit Size 1 kit
    Country of Manufacture United States
    Target for Labeling DNA, PNA, RNA
    Tag/Group Incorporated Biotin
Additional Info

    Details

    PHOTOPROBE® Reagents are nucleic acid labeling systems of choice for incorporating a label at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor creates its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.

    DNA/RNA labeling is based on aryl azide chemistry in which the reagent, when exposed to heat or light, becomes activated and incorporates into the nucleic acid without base specificity. The nucleic acid labeling reaction can be carried out using a mercury vapor bulb (sun lamp), a UV lamp which produces light in the 350 nm to 370 nm range, a halogen lamp, a heating block, or a thermal cycler providing the investigator with great flexibility in experimental design.

    A distinct advantage of the simple setup in each of these labeling options is the ease and economy of scaling up.

    PHOTOPROBE® Biotin incorporates biotin in one reaction step. PHOTOPROBE® (Long Arm) Biotin has an extra long linker arm and should be used if increased distance between the sample and the tag is needed. In a similar manner, PHOTOPROBE® Amine incorporates primary amines into nucleic acids. These amino groups can subsequently be used to attach haptens, affinity tags, or fluorochromes, or to immobilize nucleic acids to a solid matrix.

     

    PHOTOPROBE Mechanism

     

    Components of the PHOTOPROBE® (Long Arm) Biotin Labeling and Detection System:

    Kit consists of the following:

    • PHOTOPROBE® (Long Arm) Biotin Labeling Kit
    • 10x Casein Solution (250 ml concentrate)
    • Alkaline Phosphatase Streptavidin (1 ml)
    • BCIP/NBT Substrate Kit

    Contents of the PHOTOPROBE® (Long Arm) Biotin Labeling Kit :

    Each PHOTOPROBE® Biotin Labeling Kit contains the following components to label up to 250 ug of nucleic acid (or to carry out up to 50 labeling reactions):

    • PHOTOPROBE® Biotin Reagent
    • Tris Buffer
    • sec-Butanol
    • Biotinylated DNA Standard
    • Precipitant

     

     

     

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