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PHOTOPROBE® Biotin for Nucleic Acid Labeling

Catalog Number: SP-1000

Availability: In stock

$160.00
Unit Size: 1 kit

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Overview

    Features of PHOTOPROBE® Biotin:

    • A method of choice for incorporating biotin at multiple sites along the entire length of the nucleic acid. This method does not destroy the original nucleic acid nor create its copy.
    • Labels DNA, RNA or PNA. 
    • Kit labels up to 250 μg of target or up to 50 labeling reactions. 


    Kit components of PHOTOPROBE® Biotin Labeling Kit:

    • PHOTOPROBE® Biotin Reagent
    • Tris Buffer
    • sec-Butanol
    • Precipitant
    • Biotinylated DNA Standard

     

    Additional Information

    Catalog Number SP-1000
    Unit Size 1 kit
    Country of Manufacture United States
    Target for Labeling DNA, PNA, RNA
    Tag/Group Incorporated Biotin
Additional Info

    Details

    PHOTOPROBE® Reagents are nucleic acid labeling systems of choice for incorporating a label at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor creates its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.

    DNA/RNA labeling is based on aryl azide chemistry in which the reagent, when exposed to heat or light, becomes activated and incorporates into the nucleic acid without base specificity. The nucleic acid labeling reaction can be carried out using a mercury vapor bulb (sun lamp), a UV lamp which produces light in the 350 nm to 370 nm range, a halogen lamp, a heating block, or a thermal cycler providing the investigator with great flexibility in experimental design.

    A distinct advantage of the simple setup in each of these labeling options is the ease and economy of scaling up.

    PHOTOPROBE® Biotin incorporates biotin in one reaction step. PHOTOPROBE® (Long Arm) Biotin has an extra long linker arm and should be used if increased distance between the sample and the tag is needed. In a similar manner, PHOTOPROBE® Amine incorporates primary amines into nucleic acids. These amino groups can subsequently be used to attach haptens, affinity tags, or fluorochromes, or to immobilize nucleic acids to a solid matrix.

    PHOTOPROBE Mechanism

    Kit Contents:

    Each PHOTOPROBE® Biotin Kit contains the following components to label up to 250 ug of nucleic acid (or to carry out up to 50 labeling reactions):

    • PHOTOPROBE® Biotin Reagent
    • Tris Buffer
    • sec-Butanol
    • Biotinylated DNA Standard
    • Precipitant

     

     

     

    Figure Legend:

     

    CGH sum karyogram

    CGH sum karyogram was created after analyzing PHOTOPROBE labeled tumor DNA from a small cell lung cancer. Biotin was detected with Vector Fluorescein Avidin D (green). Chromosomes from 15 metaphase spreads were analyzed for each hybridization. Figure provided courtesy of Dirk Korinth, Konrad Donhuijsen, Ulrike Bockmühl, and Iver Petersen, Institute of Pathology, University Hospital Charité, Berlin, Germany.

     

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