Horse Anti-Goat IgG Antibody (H+L), Peroxidase

PI-9500-1
SKU Unit Size Price Qty
PI-9500-1 1 mg
$160.00

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Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Cross-linked with the highest specific activity horseradish peroxidase 
  • Supplied in solution

Specifications

More Information
Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C, DO NOT FREEZE
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
Recommended Usage Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in thse applications, blocking with Animal-Free blocker™ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti goat IgG is 0.5-5.0 µg/ml.
Target Species Goat
Conjugate Peroxidase
Host Species Horse
Format Concentrate

Documents

Citations

Technical Information

Our high affinity Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.

As well as recognizing goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

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