Wheat Germ Agglutinin (WGA), Peroxidase

PL-1026
SKU Unit Size Price Qty
PL-1026-2 2 mg
$228.00

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Description

Peroxidase conjugated Wheat Germ Agglutinin is specially prepared for neuronal transport studies. This product is supplied as 40 mg/ml of protein in a 2 mg package ready for injection. After tissue preparation, detection can be readily achieved with a peroxidase substrate.

Specifications

More Information
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Neurobiology, Blotting Applications, ELISAs, Glycobiology
Conjugate Peroxidase
Sugar Specificity N-Acetylglucosamine

Documents

Technical Information

Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin is used for the purification of insulin receptors and for neuronal tracing.

WGA-HRP can be used for both anterograde and retrograde tracing. Our peroxidase-wheat germ agglutinin is specially prepared for neuronal transport studies. WGA-peroxidase features a high concentration of protein and is supplied in a 2 mg package at 40 mg/ml ready for injection. Unconjugated wheat germ agglutinin, also used in neuronal studies is available as 10 mg and 25 mg of salt-free, lyophilized lectin.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

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