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NicKit p.s.o. Probe Size Optimization Kit

Catalog Number: MB-1905

Availability: In stock

$105.00
Unit Size: 1 kit

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Overview

    Features:

    NicKit™ p.s.o. has been developed for reducing the size of DNA probes to an optimal length for in situ hybridization, without the problem of over- or under- digestion encountered with other methods. The procedure involves a brief U.V. irradiation of probe DNA using a transilluminator or hand-held U.V. lamp followed by enzymatic digestion using a specially prepared endonuclease. The phosphodiester bond at pyrimidine dimers formed by U.V. irradiation is cleaved, resulting in average DNA lengths of 400 bases or less. Probe DNA can be subsequently labeled with PHOTOPROBE® Biotin or the FastTag® system. The NicKit™ p.s.o. reagents can treat up to 300 µg of DNA.

    Kit components:

    • T4 endonuclease V
    • T4 endonuclease V reaction buffer

     

    Additional Information

    Catalog Number MB-1905
    Unit Size 1 kit
    Country of Manufacture United States
    Applications In situ hybridization
Additional Info

    Details

    NicKit p.s.o. has been developed for reducing the size of DNA probes to an optimal length for in situ hybridization, without the problem of over- or under- digestion encountered with other methods. The procedure involves a brief U.V. irradiation of probe DNA using a transilluminator or hand-held U.V. lamp followed by enzymatic digestion using a specially prepared endonuclease. The phosphodiester bond at pyrimidine dimers formed by U.V. irradiation is cleaved, resulting in average DNA lengths of 400 bases or less. Probe DNA can be subsequently labeled with PHOTOPROBE Biotin or the FastTag system. The NicKit p.s.o. reagents can treat up to 300 g of DNA.
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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Protocol