NEUROBIOTIN®-PLUS Tracer

SP-1150
SKU Unit Size Price Qty
SP-1150-5 5 mg
$109.00

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Description

NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling

Features:

  • Fixable primary amine
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

More Information
Unit Size 5 mg
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage Fixable with formaldehyde or glutaraldehyde
Solution 10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide.
Molecular Weight 358
Neuronal Tracer - Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Technical Information

Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN-Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.

NEUROBIOTIN-Plus shares the same key advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde/li>

NEUROBIOTIN-Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

*Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.