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NEUROBIOTIN-Plus

Catalog Number: SP-1150

Availability: In stock

$95.00
Unit Size: 5 mg

Dendritic spines, 4h post-labeling with NEUROBIOTIN Plus.  Image kindly provided by Dr Judith Schweimer, Dept of Pharmacology, University of Oxford, U.K.
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Overview

    NEUROBIOTIN™-Plus Tracer is impervious to cleavage and breakdown by biotinidase.

    Features:

    • Biotin label with a biotinidase-resistant linkage
    • Fixable primary amine
    • Used for visualizing neural architecture and for the identification of gap junction coupling
    • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
    • Can be delivered by many routes such as intracellular electodes, microinjection, cut-loading, or scrape-loading
    • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

    Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

    • Better solubility
    • More efficiently iontophoresed
    • Remains in cell longer
    • Non-toxic
    • Can be fixed with formalin or glutaraldehyde

    Additional Information

    Catalog Number SP-1150
    Unit Size 5 mg
    Country of Manufacture United States
    Neuronal Tracer - Direction of Transport Anterograde/Retrograde
    Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence
Additional Info

    Details

    Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN™ -Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.

    NEUROBIOTIN™ -Plus shares the same key advantages of NEUROBIOTIN™ Tracer over biocytin and other neuronal labels:

    • Better solubility
    • More efficiently iontophoresed
    • Remains in cell longer
    • Non-toxic
    • Can be fixed with formalin or glutaraldehyde/li>

    NEUROBIOTIN™ -Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

    *Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Data Sheet