NEUROBIOTIN-Plus

Cat. No: SP-1150
Price: $104.00
Unit Size: 5 mg

Overview

NEUROBIOTIN™-Plus Tracer is impervious to cleavage and breakdown by biotinidase.

Features:

  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde
Cat. No. SP-1150-5
Unit Size 5 mg
Country of Manufacture United States
Neuronal Tracer - Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

More Information

Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN™ -Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.

NEUROBIOTIN™ -Plus shares the same key advantages of NEUROBIOTIN™ Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde/li>

NEUROBIOTIN™ -Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

*Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.

Protocols/Data Sheets/SDS