NEUROBIOTIN® 488 Tracer

SP-1125
SKU Unit Size Price Qty
SP-1125-2 2 mg
$118.00

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Description

NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

More Information
Unit Size 2 mg
Maximum Emission 517 nm
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage NEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO
Molecular Weight 747
Maximum Excitation 493 nm
Neuronal Tracer - Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Technical Information

NEUROBIOTIN 488 Tracer is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. NEUROBIOTIN 488 Tracer is readily fixed with standard aldehyde based fixatives. This tracer can be viewed directly under fluorescence microscopy.