NEUROBIOTIN 488

Cat. No: SP-1125
Price: $114.00
Unit Size: 2 mg

Overview

NEUROBIOTIN™ 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN™ Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde
Cat. No. SP-1125-2
Unit Size 2 mg
Country of Manufacture United States
Neuronal Tracer - Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

More Information

NEUROBIOTIN™ 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine

NEUROBIOTIN™ 488 Tracer is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. NEUROBIOTIN™ 488 Tracer is readily fixed with standard aldehyde based fixatives. This tracer can be viewed directly under fluorescence microscopy. The tracer can be detected and amplified via the biotin moiety with fluorescent or enzyme-based avidin or streptavidin detection systems.