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NEUROBIOTIN 350

Catalog Number: SP-1155

Availability: In stock

$105.00
Unit Size: 2 mg

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Overview

    NEUROBIOTIN™ 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

    Features:

    • Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
    • Biotin label with a biotinidase-resistant linkage
    • Fixable primary amine
    • Used for visualizing neural architecture and for the identification of gap junction coupling
    • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
    • Can be delivered by many routes such as intracellular electodes, microinjection, cut-loading, or scrape-loading
    • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

    Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

    • Better solubility
    • More efficiently iontophoresed
    • Remains in cell longer
    • Non-toxic
    • Can be fixed with formalin or glutaraldehyde

    Additional Information

    Catalog Number SP-1155
    Unit Size 2 mg
    Country of Manufacture United States
    Neuronal Tracer - Direction of Transport Anterograde/Retrograde
    Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence
Additional Info

    Details

    NEUROBIOTIN™ 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains:

    • a biotin label with a biotinidase-resistant linker.
    • a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor 350.
    • a fixable primary amino group.

    Similar in structure to NEUROBIOTIN™ 488, NEUROBIOTIN™ 350 is also an amine containing biotin derivative. However, it is conjugated with a bright blue fluorophore that would contrast with other green or red fluorescent markers or reagents present in the same preparation. For administration purposes NEUROBIOTIN™ 350 has a net positive charge and is highly soluble in a number of commonly used buffer and salt solutions.

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Data Sheet