NanoLINK streptavidin magnetic beads possess the highest biotin-binding capacity of any commercially available polymer-encapsulated streptavidin bead. These beads are particularly suited for high throughput robotic applications where high biotin loads must be immobilized and separated using a suitably strong magnet.
The beads can be used to immobilize:
- Biotinylated antibodies and other proteins
- Biotinylated dsDNA (gDNA, PCR products) or biotinylated aRNA
- Biotinylated oligonucleotides
The main advantage of using these ultra-high capacity beads is that it leads to a reduction in the overall particle mass required to immobilize a biotinylated sample. This in turn leads to reduced costs and lower non-specific background (NSB).
Applications include separation of biotin-labeled biomolecules such as biotin- labeled antibodies, genomic DNA, RNA, PCR products, oligonucleotides (e.g. biotinylated oligo (dT) or peptides. NanoLINK streptavidin magnetic beads are also ideal for generating single-stranded PCR templates (by removal of the unbiotinylated competing PCR strand), which allows for a dramatic increase in the efficiency of hybridization to complementary targets.
NanoLINK streptavidin magnetic beads also provide an affordable alternative for automated, high throughput immobilization processes that utilize 96-well magnets to affect multiplex binding and separation of nucleic acid or immunoassay biomolecules. NanoLINK beads are supplied at 10 mg/mL, with 1% solids, in nuclease-free water containing 0.05% sodium azide. There are no surfactants present although particles can be washed with water prior use to remove residual azide if desired.
Percent Solids: NanoLINK streptavidin magnetic beads are packaged nominally at 1% solids (10 mg/mL) as measured using spectroscopic analysis set by their optical density at 600 nm versus a known mass standard of the same size.
Biotin Binding Capacity: The biotin binding capacity of NanoLINK streptavidin magnetic beads is measured in nmol/mg. Biotin binding is quantitatively measured by incubating a known mass of beads (0.5 mg) with a fluorescein-biotin standard solution for 60 minutes and quantifying the amount of residual unbound fluorescein-biotin left in solution versus negative control beads.
Size Distribution by Scanning Electron Microscopy (SEM): Scanning electron microscopy confirms a bimodal size distribution for NanoLink Magnetic Beads. The streptavidin beads consist of a core polymeric particle ~ 850 nm surrounded by a population of similar but smaller polymeric beads ~ 150 nm.
Quality control: A sample of each NanoLINK batch is retained for biotin binding capacity and % solids analysis. The remainder is packaged, refrigerated, and preserved in nuclease-free water containing 0.05% sodium azide to prevent microbial contamination.
Cleaning: Surfactants are not added to this product and the particles are thoroughly washed with nuclease-free water containing 0.05% sodium azide prior to packaging. For some applications it may be desirable to remove residual azide using a brief wash.
Stability: Particles should be stored at 2-8o C. Do not freeze. If beads are settled, resuspend by suitable methods including: vortexing, rotary mixing, or swirling. NanoLINK streptavidin magnetic beads remain stable when stored at 2-8o C for 1 year.
Washing: NanoLINK streptavidin magnetic beads are washed by magnetic separation using commercially available magnetic stands. Magnetic stands are available in 50 mL, 15 mL, 1.5 mL, and 96-well plate formats from various vendors. NanoLINK beads are placed on a magnetic stand for 2-3 minutes and the clarified supernatant removed without disturbing the pellet.
Re-suspension: After long-term storage and settling of beads, it is best to resuspend the beads thoroughly to avoid any mild bead-to-bead aggregation.
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