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Mouse on Mouse (M.O.M.™) ImmPRESS™ HRP (Peroxidase) Polymer Kit

Catalog Number: MP-2400

Availability: In stock

$270.00
  • Buy 10 for $243.00 each and save 10%
  • Buy 30 for $216.00 each and save 20%
Unit Size: 1 kit

Left, Without M.O.M.:  mouse intestine stained with standard anti-mouse IgG polymer system and Vector DAB.  Hematoxylin counterstain.  Note background IgG staining.  Right, With M.O.M.:  Vector M.O.M. ImmPRESS Kit, Vector DAB and no primary. Zoom

Overview

    Features:

    • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
    • Clear, crisp specific staining of antigens of interest
    • Based on ImmPRESS™ reagent protocols
    • Procedures are simple and easy to follow
    • No tedious calculations or antibody prebinding steps required

    Kit Contents:

    • 0.6 ml concentrated stock solution of M.O.M.™ Mouse Ig Blocking Reagent
    • 30 ml ready-to-use (R.T.U.) 2.5% Normal Horse Serum (NHS) for general protein blocking
    • 15 ml R.T.U. M.O.M.™ ImmPRESS™ Anti-Mouse Ig Reagent (made in horse, ready-to-use)

    Additional Information

    Catalog Number MP-2400
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry
    Technology Micropolymer Reagents, Mouse on Mouse (M.O.M.) Systems
    Detection Enzyme Peroxidase
    Conjugate Micropolymer HRP
    Target Species Mouse
    Blocking Action Endogenous Mouse Ig
Additional Info

    Details

    For years, researchers attempting to localize mouse primary antibodies on mouse tissue have relied on the Vector® M.O.M.™ Kit to produce crisp, strong, specific staining of antibody targets.  The Vector® M.O.M.™ Kits are specifically designed to significantly reduce endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue.  The inability of an anti-mouse detection antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining.  This problem can be essentially eliminated by using Vector® M.O.M.™ Kits.  The result is clear, crisp staining of the antigens of interest.  Tedious calculations and primary antibody pre-binding steps are not required.

    This Vector® M.O.M.™ ImmPRESS™ Kit contains our proprietary M.O.M.™ Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step M.O.M.™ ImmPRESS™ Peroxidase Polymer reagent.  The M.O.M.™ ImmPRESS™ HRP polymer reagent is affinity purified and specifically optimized for use with the M.O.M.™ Mouse Ig Blocking Reagent.  The highly active peroxidase micropolymers of the ImmPRESS™ Detection System provide outstanding sensitivity, limit steric interference, and enhance accessibility.  Excellent staining results for a once difficult application have now become routine with the Vector® M.O.M.™ System.

    Features of the Vector® M.O.M.™ ImmPRESS™ HRP Polymer Kit:

    • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
    • Clear, crisp specific staining of antigens of interest
    • Simple, easy procedure
    • Based on ImmPRESS™ reagent protocols
    • No tedious calculations required

    Key advantages of the ImmPRESS™ Polymer System:

    • High sensitivity
    • Low background
    • Micropolymer technology
    • Enhanced accessibility to nuclear and membrane antigens
    • One-step detection
    • Ready-to-use in a convenient dropper bottle
    • Shorter assay time
    • Simplified multiple labeling

    Kit Contents:

    • 0.6ml M.O.M.™ Mouse Ig Blocking Reagent (concentrate)
    • 30 ml R.T.U. 2.5% Normal Horse Serum
    • 15 ml R.T.U. M.O.M.™ ImmPRESS™ Anti-Mouse Ig Reagent

    The Vector® M.O.M.™ ImmPRESS™ Kit (Cat. No. MP-2400) contains sufficient reagents to stain approximately 150 mouse sections.

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