M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein

FMK-2201
SKU Unit Size Price Qty
FMK-2201 1 kit
$335.00

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Description

 

The Vector M.O.M. Fluorescein Kit is specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. It contains a proprietary Mouse IgG Blocking Reagent, a M.O.M. Biotinylated Anti-Mouse Ig Reagent, and Fluorescein Avidin DCS and is based on a simple and easy to follow avidin/biotin protocol.

Key advantages of the Vector M.O.M. Immunodetection Kits:

  • Clear, crisp specific staining of antigens of interest
  • Based on VECTASTAIN® ABC reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

Kit Contents:

  • 6 ml of M.O.M. Protein Concentrate
  • 1 ml M.O.M. Mouse Ig Blocking Reagent
  • 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
  • 0.4 ml Fluorescein Avidin DCS

The Vector® M.O.M. Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections.

Specifications

More Information
Unit Size 1 kit
Applications Immunofluorescence
Technology ABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems
Conjugate Fluorescein
Color of Fluorescence Green
Target Species Mouse
Format Concentrate
Blocking Action Endogenous Mouse Ig

Documents

Product FAQs

For my IHC application, I intend to apply a mouse IgG primary antibody on rat tissue sections. Should I use the Vector Mouse on Mouse detection kit?

Vector Laboratories’ Mouse on Mouse detection kits (M.O.M.®) are designed for the detection of mouse IgG primary antibodies on mouse tissue sections. For detecting mouse primary antibodies on rat tissue, we offer a selection of anti-mouse, rat adsorbed secondary detection reagents. These are intended to be used for this application and would generate the most optimal signal to noise staining ratio. We offer a biotinylated anti-mouse IgG, rat adsorbed secondary antibody (Cat. No. BA-2001) for use with VECTASTAIN® ABC kits or avidin and streptavidin enzyme conjugates. Alternatively, we offer an ImmPRESS® HRP polymer anti-mouse IgG, rat adsorbed detection kit (Cat. No. MP-7422) for a convenient, one-step IHC methodology.

Technical Information

The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining.  This problem can be essentially eliminated by using a Vector M.O.M. Kit.  The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.

The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, Discovery Through Color: A Guide to Multiple Antigen Labeling , available on our website.

Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.

The Vector M.O.M. ImmPRESS® Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS™ Peroxidase Polymer reagent.