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Mouse on Mouse (M.O.M.™) Biotinylated Anti-Mouse Ig Reagent

Catalog Number: MKB-2225

Availability: In stock

$115.00
Unit Size: 0.1 ml

Small Bowel (frozen, double label): PGP9.5 (m), MOM Basic Kit, VECTASTAIN ABC AP Standard Kit, Vector Red substrate (red); Desmin (m), MOM Fluorescein Kit (green). Zoom

Overview

    Mouse on Mouse (M.O.M.™) Biotinylated Anti-Mouse Ig Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector® M.O.M.™ Immunodetection Kit components.  This reagent is supplied as 0.1 ml stock solution sufficient to make 25 ml of working solution.

    Features of the M.O.M.™ Immunodetection system:

    • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
    • Clear, crisp specific staining of antigens of interest
    • Based on VECTASTAIN® ABC or ImmPRESS™ reagent protocols
    • Procedures are simple and easy to follow
    • No tedious calculations or antibody prebinding steps required

    Product Citations:

    Additional Information

    Catalog Number MKB-2225
    Unit Size 0.1 ml
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications
    Target Species Mouse
    Conjugate Biotinylated
    Format Concentrate
Additional Info

    Details

    M.O.M.™ Biotinylated Anti-Mouse Ig Reagent (MKB-2225) is a specially modified secondary antibody that has been optimized specifically for use with the Vector® M.O.M.™ Immunodetection Kit components. This is the same reagent contained in the Vector® M.O.M.™ Kits (Cat. Nos. PK-2200, FMK-2201, and BMK-2202).

    The Vector® M.O.M.™ Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector® M.O.M.™ Kit. The result is clear, crisp specific staining of the antigens of interest.  Excellent staining results for a once difficult application have now become routine with the Vector® M.O.M.™ System.

    Key advantages of the Vector M.O.M.™ Immunodetection Kits:

    • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
    • Clear, crisp specific staining of antigens of interest
    • Based on VECTASTAIN® ABC reagent protocols
    • Procedures are simple and easy to follow
    • No tedious calculations or antibody prebinding steps required

    The Vector M.O.M.™ Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

    The M.O.M.™ Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, "Discovery Through Color: A Guide to Multiple Antigen Labeling ", available on our website.

    Four Vector® M.O.M.™ Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M.™ Mouse Ig Blocking Reagent and the M.O.M.™ Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M.™ Mouse Ig Blocking Reagent and M.O.M.™ Biotinylated Anti-Mouse Ig Reagent are also available separately.

    The Vector® M.O.M.™ ImmPRESS™ Kit contains our proprietary M.O.M.™ Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M.™ ImmPRESS™ Peroxidase Polymer reagent.

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