MagnaLINK® 4FB Magnetic Beads (2.8 µm)

M-1004-010
SKU Unit Size Price Qty
M-1004-010 1 mL
$416.00

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Description

MagnaLINK 2.8 µm 4FB Magnetic Beads provide a high surface area activated with the 4FB linker to enable easy covalent immobilization for user-defined, high-performance affinity purification schemes. They are colloidally stable with or without non-ionic detergents. The 4FB linker enables easy and efficient immobilization of any biomolecule modified with the complementary HyNic linker (Cat. No. S-1002-105) using ChromaLINK® technology. HyNic-modifed peptides, oligonucleotides, and antibodies rapidly form a stable conjugate bond with MagnaLINK 4FB Magnetic Beads. The MagnaLINK 4FB Magnetic Beads are validated and optimized for use with high-throughput magnetic platforms as well as for benchtop magnetic stands.

Specifications

More Information
Bead Size 2.8 µm
Loading Capacity ≥ 25 nmol/mg
Reactivity HyNic
Applications Antibody Labeling, Aptamers, Next-Generation Sequencing (NGS), Photocrosslinking Studies
Recommended Storage 2° - 8°C – Do Not Freeze

Documents

Technical Information

Product Description

MagnaLINK 4FB magnetic microspheres are uniform, polymer encapsulated, nanometer-sized, super-paramagnetic particles activated with 4 formyl benzamide functionalities (4FB) see Figure 1. The aromatic aldehydes are introduced to the hydrophilic amino-modified surface by reaction with sulfo-succinymidly 4-formylbenzoate (Sulfo-S-4FB).

The high surface area and low non-specific binding of MagnaLINK 4FB magnetic microspheres are ideal for covalent immobilization of aromatic hydrazine-modified antibodies, peptides, DNA, RNA, oligonucleotides and/or oligosaccharides. The paramagnetic properties of these microspheres allow them to be used for the rapid separation of bound from unbound biomolecules using a suitably strong magnet.

Figure 1. MagnaLINK 4FB Magnetic Microspheres.

Features:

  • High surface area (2.8 ± 0.2 micron diameter)

  • Consistent aromatic aldehyde loading capacities (30-45 nmol/mg)

  • Encapsulated (no exposed iron) monodisperse microspheres

  • Fast magnetic response time (25 electromagnetic units)

  • 4FB is stable in aqueous solution for months at 4°C

  • Immobilizes approximately 120 ug IgG per mg of solid phase

  • Paramagnetic (no residual magnetism)

    % Solids: MagnaLINK 4FB magnetic microspheres are packaged at nominally 1% solids (10 mg/mL) as measured using spectroscopic analysis which is set by their optical density at 600 nm against a microsphere size standard.

    Aromatic Aldehyde (4FB) Functional Capacity: The aromatic aldehyde (4FB) surface capacity of each lot of particles is quantified by measuring the amount of unbound HyNic-peptide (OD280) left in solution versus an unmodified control microsphere after a 2 hour incubation. Refer to Certificate of Analysis for the specific 4FB functional capacity of each lot (nmol/mg).

Intellectual Property

SoluLINK® Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.

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