The ImmPRESS™ Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS™ Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS™ Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.
ImmPRESS™ VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems. ImmPRESS™ VR Polymer HRP Anti-Rabbit IgG is designed to be used on the following tissues: bovine, goat, sheep, swine, horse, cat, dog, rat and mouse (as well as human).
Key advantages of the ImmPRESS™ VR Polymer System:
- High sensitivity
- Low background
- Micropolymer technology
- Enhanced accessibility to nuclear and membrane antigens
- One-step detection
- Ready-to-use in a convenient dropper bottle
- Shorter assay time
- Simplified multiple labeling
Staining Procedure
The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
Learn more about the ImmPRESS™ Polymer Detection Systems in double labeling.
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