ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent

MP-6402-15
SKU Unit Size Price Qty
MP-6402-15 15 ml
$183.00

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Description

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.

ImmPRESS VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Minimal cross-reactivity with bovine, goat, sheep, pig, horse, cat, dog, and rabbit (as well as human). For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Specifications

More Information
Unit Size 15 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Mouse
Format Ready-to-Use

Kit Components

Kit Contents:

  • ImmPRESS VR Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)
  • The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Documents

Product FAQs

What are the advantages of using ImmPRESS® Excel kits compared with one-step peroxidase polymer detection systems?

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Our research lab has an open automated staining platform. Do you know if the ImmPRESS reagents can be applied to an automated system, and if so, do you have any guidelines or procedures?

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://info.vectorlabs.com/l/360741/2019-01-11/2hv9mw

Technical Information

For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS Reagents in Single Labeling 

Learn more about the ImmPRESS Polymer Detection Systems in double labeling.

Product review on Biocompare.com.