The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product.
High sensitivity and low background
Enhanced accessibility to nuclear and membrane antigens
Ready-to-use in a convenient dropper bottle
Shorter assay time
Simplified multiple labeling
Designed for use with both rabbit and mouse primary antibodies
The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.
Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.
Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
15 ml BLOXALL Endogenous Enzyme Blocking Solution
ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)
The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.
The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
Consider Species Cross-Reactivity
When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:
Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.