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ImmPRESS™ HRP Anti-Goat IgG (Peroxidase) Polymer Detection Kit, made in Horse

Catalog Number: MP-7405

Availability: In stock

Product Name Unit Size Price Qty
ImmPRESS HRP Anti-Goat IgG (Peroxidase) Polymer Detection Kit, made in Horse 15 ml
$130.00
ImmPRESS HRP Anti-Goat IgG (Peroxidase) Polymer Detection Kit, made in Horse 50 ml
$340.00

Prostate:  Prostate Specific Antigen (p), ImmPRESS Anti-Goat Ig Kit, ImmPACT DAB (brown), substrate.  Hematoxylin QS counterstain. Zoom

Overview

    Features:

    • High sensitivity
    • Low background
    • Micropolymer technology
    • Enhanced staining of nuclear and membrane antigens
    • One-step detection
    • Ready-to-use in a convenient dropper bottle
    • Shorter assay time
    • Simplified multiple labeling
    • Suitable for use with goat, sheep, and bovine IgG primary antibodies

    Kit Contents:

    • ImmPRESS™ (Peroxidase) Polymer Anti-Goat IgG Reagent (made in horse, ready-to-use)
    • 2.5% Normal Horse Serum for blocking (ready-to-use)

    The ImmPRESS™ (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS™ (50 ml) Kit will stain approximately 250-500 sections.

    Additional Information

    Catalog Number MP-7405
    Unit Size 15 ml, 50 ml
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
    Technology Micropolymer Reagents
    Detection Enzyme(s) Peroxidase
    Conjugate Micropolymer HRP
    Target Species Goat
    Format Ready-to-Use
Additional Info

    Details

    The ImmPRESS™ polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

    The peroxidase micropolymers of the ImmPRESS™ HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

    Key advantages of the ImmPRESS™ Polymer System:

    • High sensitivity
    • Low background
    • Micropolymer technology
    • Enhanced accessibility to nuclear and membrane antigens
    • One-step detection
    • Ready-to-use in a convenient dropper bottle
    • Shorter assay time
    • Simplified multiple labeling
    • Suitable for use with goat, sheep, and bovine IgG primary antibodies

    Staining Procedure

    The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

    ImmPRESS Reagents in Single Labeling

    Consider Species Cross-Reactivity

    When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

    • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS™ Anti-Mouse IgG, Rat Adsorbed).
    • Use the M.O.M. ImmPRESS™ Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

     

    Learn more about the ImmPRESS™ Polymer Detection Systems in double labeling.

    Product review on Biocompare.com.

     
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