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ImmPRESS Duet Double Staining HRP/AP Polymer Kit (anti-rabbit IgG-brown, anti-mouse IgG-red)

Catalog Number: MP-7714

Availability: In stock

$445.00
Unit Size: 1 kit

Colon Carcinoma (paraffin): ImmPRESS Duet Kit (MP-7724) used to detect mouse anti-CD34 (ImmPACT DAB, brown) and rabbit anti-Ki67 (ImmPACT Vector® Red, magenta) antibodies. Zoom

Overview

    Features:

    Save time and streamline your double staining IHC workflow with the ImmPRESS™ Duet Double Staining Polymer Kit.

    • Complete Kits for IHC Double Labeling
    • Convenient Dual Specificity (anti-mouseIgG/rabbit IgG) ImmPRESS™ HRP/AP Polymer Reagent
    • High sensitivity and Specificity
    • Non-Biotin Based Reagents
    • Prediluted, Ready-to-use

    Kit Contents:

    • 15 ml BLOXALL™ Blocking Solution
    • 15 ml 2.5% Normal Serum
    • 15 ml ImmPRESS™ Duet HRP/AP Polymer Reagent (mixture of ImmPRESS™ HRP Anti-Rabbit IgG and ImmPRESS™ AP Anti-Mouse IgG)
    • ImmPACT™ DAB EqV Substrate (HRP, brown)
    • ImmPACT™ Vector® Red Substrate (AP, magenta)

    Additional Information

    Catalog Number MP-7714
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry
    Technology Micropolymer Reagents
    Detection Enzyme(s) HRP/AP
    Conjugate Micropolymer HRP/Micropolymer AP
    Target Species Universal (Mouse/Rabbit)
    Format Ready-to-Use
    Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking
Additional Info

    Details

    Save time and streamline your double staining IHC workflow with the ImmPRESS™ Duet Double Staining Polymer Kit. This kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS™ micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies. These reagents are then blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT™ DAB EqV (HRP, brown) and ImmPACT™ Vector® Red (AP, magenta) substrates are included to complete this easy to use staining kit.

    Key advantages

    • Complete double staining HRP/AP Kits
    • Highly cross-adsorbed, non-biotin polymer detection reagent
    • High sensitivity and specificity
    • Prediluted, ready- to-use in convenient dropper bottles

    Kit includes

    1. 15 ml BLOXALL™ Blocking Solution
    2. 15 ml 2.5% Normal Serum
    3. 15 ml ImmPRESS™ Duet HRP/AP Polymer Reagent (mixture of ImmPRESS™ HRP Anti-Rabbit IgG and ImmPRESS™ AP Anti-Mouse IgG)
    4. ImmPACT™ DAB EqV Substrate (HRP, brown)
    5. ImmPACT™ Vector® Red Substrate (AP, magenta)

    The ImmPRESS™ Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens. Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

    Also included, BLOXALL™ Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

    All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

     

    Learn more about the ImmPRESS™ Polymer Detection Systems in double labeling.

    Product review on Biocompare.com.

     

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Protocol