ImmPACT® DAB Peroxidase (HRP) Substrate

Cat. No: SK-4105
Price: $152.00
Unit Size: 120 ml


ImmPACT DAB is an entirely new formula for a diaminobenzidine-based peroxidase substrate. ImmPACT DAB produces a dark brown reaction product that is crisper and is 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both manual and automated staining methods. The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C. This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB. This product can also be used on blots.

DAB (3,3'-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).


  • 2-4 fold greater sensitivity than original substrate kit
  • Optimized for IHC/ICC/ISH and blots
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Working solutions stable for up to 14 days at 4 °C
  • Supplied as 120 ml of diluent and concentrated DAB stock solution
Cat. No. SK-4105
Unit Size 120 ml
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Brown
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

More Information

Relative Sensitivity of Substrates

Substrate Combinations


Counterstain Compatibility


Safety Information

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