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Immunohistochemistry Resource Guide

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Table of Contents

Multiple Antigen Labeling Simplified

A key advantage of the ImmPRESS® Polymer Reagent is that it significantly shortens staining times for multiplex immunohistochemistry (IHC) protocols.

  • Fewer steps than conventional protocols decreases slide handling.
  • Eliminates the need for avidin/biotin blocking steps in samples with endogenous biotin.
Multiple Antigen Label Breast Carcinoma Multiple Antigen Label Breast Carcinoma Multiple antigen label breast carcinoma
Breast Carcinoma: • Ki67 (rm), ImmPRESS Reagent (HRP; Universal), Vector® DAB (brown) • CD34, ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Multiple Antigen Label Colon Multiple Antigen Label Colon Multiple antigen label colon
Colon: • M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector Blue (blue) • CD20 (m), ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Multiple Antigen Label Colon Multiple Antigen Label Colon Multiple antigen label colon
Colon: • CD3 (rm), ImmPRESS Reagent (HRP) Anti-Rabbit IgG, ImmPACT AMEC Red • CD34 (m), ImmPRESS-AP Anti-Mouse IgG Reagent, Vector Blue (blue).

Using the ImmPRESS® Polymer Kits for multiplex IHC protocols.

1 Add First Primary Antibody

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Multiple antigen label immpress

2 Add ImmPRESS Reagent

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Multiple antigen label immpress

3 Add Enzyme Substrate I

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Multiple antigen label immpress

4 Add Second Primary Antibody

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Multiple antigen label immpress

5 Add ImmPRESS Reagent

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Multiple antigen label immpress

6 Add Enzyme Substrate II

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Multiple antigen label immpress

Read more about multiplex IHC protocols: Discovery Through Color: IHC Multiplexing Guide