Vector Labs is closed to observe Independence Day (US Holiday) on Thursday, July 4th.
We will be back in the office on Friday. We will respond to emails upon our return. Have a wonderful day.

Immunohistochemistry Resource Guide

Not enough time read now ?

Ihc Guide Cover V Ihc Guide Cover V Vectorlabs Ihc guide cover v Vectorlabs

Table of Contents

Multiple Antigen Labeling Simplified

A key advantage of the ImmPRESS® Polymer Reagent is that it significantly shortens staining times for multiplex immunohistochemistry (IHC) protocols.

  • Fewer steps than conventional protocols decreases slide handling.
  • Eliminates the need for avidin/biotin blocking steps in samples with endogenous biotin.
Multiple Antigen Label Breast Carcinoma Multiple Antigen Label Breast Carcinoma Vectorlabs Multiple antigen label breast carcinoma Vectorlabs
Breast Carcinoma: • Ki67 (rm), ImmPRESS Reagent (HRP; Universal), Vector® DAB (brown) • CD34, ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Multiple Antigen Label Colon Multiple Antigen Label Colon Vectorlabs Multiple antigen label colon Vectorlabs
Colon: • M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector Blue (blue) • CD20 (m), ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Multiple Antigen Label Colon Multiple Antigen Label Colon Vectorlabs Multiple antigen label colon Vectorlabs
Colon: • CD3 (rm), ImmPRESS Reagent (HRP) Anti-Rabbit IgG, ImmPACT AMEC Red • CD34 (m), ImmPRESS-AP Anti-Mouse IgG Reagent, Vector Blue (blue).

Using the ImmPRESS® Polymer Kits for multiplex IHC protocols.

1 Add First Primary Antibody

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Vectorlabs Multiple antigen label immpress Vectorlabs

2 Add ImmPRESS Reagent

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Vectorlabs Multiple antigen label immpress Vectorlabs

3 Add Enzyme Substrate I

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Vectorlabs Multiple antigen label immpress Vectorlabs

4 Add Second Primary Antibody

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Vectorlabs Multiple antigen label immpress Vectorlabs

5 Add ImmPRESS Reagent

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Vectorlabs Multiple antigen label immpress Vectorlabs

6 Add Enzyme Substrate II

Multiple Antigen Label Immpress Multiple Antigen Label Immpress Vectorlabs Multiple antigen label immpress Vectorlabs

Read more about multiplex IHC protocols: Discovery Through Color: IHC Multiplexing Guide