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Immunohistochemistry Resource Guide

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IHC Guide Cover v1

Table of Contents

Multiple Antigen Labeling Simplified

A key advantage of the ImmPRESS® Polymer Reagent is that it significantly shortens staining times for multiplex immunohistochemistry (IHC) protocols.

  • Fewer steps than conventional protocols decreases slide handling.
  • Eliminates the need for avidin/biotin blocking steps in samples with endogenous biotin.
multiple antigen label breast carcinoma
Breast Carcinoma: • Ki67 (rm), ImmPRESS Reagent (HRP; Universal), Vector® DAB (brown) • CD34, ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
multiple antigen label colon
Colon: • M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector Blue (blue) • CD20 (m), ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
multiple antigen label colon2
Colon: • CD3 (rm), ImmPRESS Reagent (HRP) Anti-Rabbit IgG, ImmPACT AMEC Red • CD34 (m), ImmPRESS-AP Anti-Mouse IgG Reagent, Vector Blue (blue).

Using the ImmPRESS® Polymer Kits for multiplex IHC protocols.

1 Add First Primary Antibody

multiple antigen label immpress1

2 Add ImmPRESS Reagent

multiple antigen label immpress2

3 Add Enzyme Substrate I

multiple antigen label immpress3

4 Add Second Primary Antibody

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5 Add ImmPRESS Reagent

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6 Add Enzyme Substrate II

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Read more about multiplex IHC protocols: Discovery Through Color: IHC Multiplexing Guide