Immunohistochemistry Resource Guide

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Multiple Antigen Labeling Simplified

A key advantage of the ImmPRESS® Polymer Reagent is that it significantly shortens staining times for multiple antigen labeling (multiplexing).

  • Fewer steps than conventional protocols decreases slide handling.
  • Eliminates the need for avidin/biotin blocking steps in samples with endogenous biotin.

Breast Carcinoma: • Ki67 (rm), ImmPRESS® Reagent (HRP; Universal), Vector® DAB (brown) • CD34, ImmPRESS® Reagent (HRP; Universal), Vector® VIP (purple).

Colon: • M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector® Blue (blue) • CD20 (m), ImmPRESS® Reagent (HRP; Universal), Vector® VIP (purple).

Colon: • CD3 (rm), ImmPRESS® Reagent (HRP) Anti-Rabbit IgG, ImmPACT™ AMEC Red • CD34 (m), ImmPRESS®-AP Anti-Mouse IgG Reagent, Vector® Blue (blue).

Using the ImmPRESS® Polymer Kits for multiple antigen labeling

1 Add First Primary Antibody
2 Add ImmPRESS® Reagent
3 Add Enzyme Substrate I
4 Add Second Primary Antibody
5 Add ImmPRESS® Reagent
6 Add Enzyme Substrate II

Read more: Discovery Through Color: A Guide to Multiple Antigen Labeling (