Immunohistochemistry Resource Guide

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ImmPRESS™ Two-Step Amplified Polymer Systems

ImmPRESS™ Excel Amplified Polymer Staining Systems

ImmPRESS™ Excel Amplified Peroxidase (HRP) Polymer Staining Systems are complete staining kits that capitalize on all the advantages of the ImmPRESS™ HRP Polymer System technology and offer additional sensitivity and convenience.

This system employs a ready-to-use (R.T.U.) Amplifier Antibody, followed by an ImmPRESS™ Excel HRP Polymer Reagent. These reagents are affinity-purified and extensively cross-adsorbed to ensure high sensitivity and low background. The included ImmPACT™ DAB EqV (equal volume) substrate produces a crisp, dark brown reaction product with excellent sensitivity that is characteristic of the ImmPRESS™/ImmPACT™ combination.

The ImmPRESS™ Excel Amplified HRP Kits are recommended for applications that require detection of weakly-expressed antigens, in cases of unknown expression levels such as gene knock-in studies, or in determining up-regulation of a given target. This straightforward kit format yields reliable, consistent results and saves time in trying to establish optimal titrations with concentrated detection reagents. Each ImmPRESS™ Excel Amplified Staining Kit includes the following:

  • BLOXALL™ Endogenous Enzyme Blocking Solution
  • 2.5% Normal Horse Serum
  • Amplifier Antibody (goat anti-rabbit IgG or goat anti-mouse IgG)
  • ImmPRESS™ Excel Polymer Detection Reagent (horse anti-goat IgG)
  • ImmPACT™ DAB EqV Substrate (Chromogen and Buffer)

Using the ImmPRESS™ Two-Step Amplified Polymer Systems

1 Add Primary Antibody
2 Add Amplifier Antibody
3 Add ImmPRESS™ Excel HRP Polymer Reagent
4 Add Enzyme Substrate