Immunohistochemistry Resource Guide

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Customizing your VECTASTAIN® ABC Kit

If a VECTASTAIN® ABC system is not available with a biotinylated secondary antibody of your required specificity, you
can custom-build the exact kit that you require. All of our biotinylated, affinity-purified secondary antibodies (p. 38-39)
are designed for use with VECTASTAIN® ABC Standard Kits and the appropriate blocking serum. Our mix-and-match kit
components allow you to both design a custom kit to suit your needs and to use kit components interchangeably.
The reagents can be purchased individually, allowing you to combine them to suit your specific needs.

For example, to make a VECTASTAIN® Elite ® ABC Kit for use with a mouse IgG primary antibody on rat tissues:

  1. Choose the VECTASTAIN® ABC Kit that contains the desired detection enzyme but with no secondary antibody
    (e.g. VECTASTAIN® Elite ® ABC Kit, Standard).
  2. Choose the biotinylated secondary antibody (e.g. biotinylated horse anti-mouse IgG, rat adsorbed).
  3. Choose the blocking solution. We recommend a serum from the same species as the secondary antibody. (In our
    example, normal horse serum). Alternatively, select our animal-free blocking reagents for multiple antigen labeling
    (multiplex) IHC applications where antibodies from different species and a variety of detection systems are used
    on the same tissue section.

1. Choose Standard VECTASTAIN® ABC Kit with the appropriate detection enzyme

EnzymeProductCatalog Number
Peroxidase VECTASTAIN® Elite® ABC Kit PK-6100
Peroxidase R.T.U. VECTASTAIN® Elite® ABC Reagent PK-7100
Peroxidase VECTASTAIN® ABC Kit PK-4000
Alkaline Phosphatase VECTASTAIN® ABC-AP Kit AK-5000

2. Choose the biotinylated secondary antibody*

ProductConcentrateR.T.U.     
Anti-Goat IgG (H+L) made in rabbit, biotinylated BA-5000
Anti-Goat IgG (H+L) made in horse, biotinylated BA-9500 BP-9500
Anti-Human IgG (H+L) made in goat, biotinylated BA-3000
Anti-Mouse IgG (H+L) made in horse, biotinylated BA-2000 BP-2000
Anti-Mouse IgG (H+L) made in horse, rat adsorbed, biotinylated BA-2001
Anti-Mouse IgG (H+L) made in goat, biotinylated BA-9200 BP-9200
Anti-Mouse IgM (H+L) μ chain specific, made in goat, biotinylated BA-2020
Anti-Rabbit IgG (H+L) made in goat, biotinylated BA-1000 BP-9100
Anti-Rabbit IgG (H+L) made in horse, biotinylated BA-1100 BP-1100
Anti-Rat IgG (H+L) made in rabbit, biotinylated BA-4000
Anti-Rat IgG (H+L) made in rabbit, mouse adsorbed, biotinylated BA-4001
Anti-Rat IgG (H+L) made in goat, biotinylated BA-9400 BP-9400
Anti-Rat IgG (H+L) made in goat, mouse adsorbed, biotinylated BA-9401
Universal Anti-Mouse/Rabbit IgG (H+L) made in horse, biotinylated BA-1400 BP-1400
Universal Pan-Specific Anti-Mouse/Rabbit/Goat IgG (H+L) made in horse, biotinylated BA-1300

3. Choose the blocking solution

ProductConcentrateR.T.U.     
Normal Goat Serum S-1000 S-1012
Normal Rabbit Serum S-5000
Normal Horse Serum S-2000 S-2012
Animal-Free Block and Diluent SP-5030 SP-5035

* For a complete list of all biotinylated secondary antibodies please visit: https://vectorlabs.com/b-2nd-abs/
† Ready-to-use, prediluted stabilized solutions.

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