Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.
| Unit Size | 1 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 1 mg active conjugate/ml |
| Recommended Storage | 2-8 °C, DO NOT FREEZE |
| Solution | 10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal |
| Recommended Usage | Moles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 - 5.0 µg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. |
| Detection Target | Biotin |
| Conjugate | Peroxidase |
| Host Species | Goat |
| Format | Concentrate |
Biotin (Long Arm) Hydrazide
Catalog #:
SP-1100
Biotin (Long Arm) NHS
Catalog #:
SP-1200
Biotin (Long Arm) NHS, Water Soluble
Catalog #:
SP-1210
Goat Anti-Biotin, Unconjugated
Catalog #:
SP-3000
Goat Anti-Biotin, Alkaline Phosphatase Conjugated
Catalog #:
SP-3020
Goat Anti-Biotin, Fluorescein Conjugated
Catalog #:
SP-3040
Our high affinity polyclonal anti-biotin antibody is produced by hyperimmunizing goats with biotin and is purified by affinity chromatography. Anti-Biotin is available unconjugated, bound to heat stable, cross-linked agarose beads or labeled with enzymes or fluorescein. Agarose bound Anti-Biotin contains 1 mg of Anti-Biotin per ml of settled 4% agarose gel. This product can be used to bind and dissociate biotinylated molecules. Fluorescein labeled Anti-Biotin has been optimally conjugated to provide a bright fluorescent signal. The horseradish peroxidase and alkaline phosphatase conjugates have been produced with the highest specific activity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody was chosen to provide stable, highly active conjugates for immunohistochemical, enzyme immunoassay, or transfer blot applications.
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