“Differentiating leaving the nest”. Human brain tumor cancer stem cells grow in culture as sphere called “neurosphere” and can give rise to all cell types from the nervous system but in their tumoral version. Neurosphere growth allows the maintenance of stem cell characteristics (green/yellow staining, stem cell marker: Glial Fibrillary Acidic Protein). Away from the sphere, brain tumor cancer stem cells start to differentiate, here into neurons (red staining, neuronal marker: Beta-III tubulin). Cell nuclei are stained in blue (DAPI). (magnification X100). Image captured by Nicolas Skuli, Abramson Family Cancer Research Institute, University of Pennsylvania, USA. The author would like to thank Dr. Caroline Delmas for helpful technical comments and suggestions.
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