Alan Prescott, University of Dundee, UK
Images taken from a frozen section of an eye from the mitoQC mouse (McWilliams et al. J Cell Biol. 2016 Aug1;214(3):333-45) at developmental stage E16.5. Mitochondria are labelled with GFP(Green) and mCherry(Red). Nuclei are blue. Section mounted in VECTASHIELD Mounting Media. Large red dots are mitochondria in mito-lysosomes demonstrating turn-over of damaged or worn out mitochondria in active tissues-in this case the developing eye. The acidic environment of the lysosomes quenches the GFP fluorescence.
Products used: H-1000