|Unit Size||0.5 mg|
|Country of Manufacture||United States|
|Applications||Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation|
|Color of Fluorescence||Green|
The anti-rat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).
Fluorescein Rabbit Anti-Rat IgG (H+L) Antibody, mouse adsorbed, can be used for flow cytometry or for tissue staining. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining. Maximum excitation is at 495 nm and maximum emission is at 515 nm.
This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The mouse adsorbed anti-rat IgG (H+L) products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross-react with mouse immunoglobulins. This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues.