Rabbit Anti-Rat IgG Antibody, mouse adsorbed (H+L), Fluorescein

FI-4001
SKU Unit Size Price Qty
FI-4001-.5 0.5 mg
$178.00

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Description

Fluorescein Rabbit Anti-Rat IgG (H+L) Antibody, mouse adsorbed, can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with fluorescein to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 495 nm 
  • Emission: 515 nm 
  • Color: Green 
  • The mouse adsorbed anti-rat IgG (H+L) antibodies are designed for use with mouse tissues that may contain endogenous mouse immunoglobulins.

Specifications

More Information
Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.0 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 510-520 nm
Maximum Excitation 490-500 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Target Species Rat
Conjugate Fluorescein
Color of Fluorescence Green
Host Species Rabbit
Format Concentrate

Documents

Technical Information

The anti-rat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

Fluorescein Rabbit Anti-Rat IgG (H+L) Antibody, mouse adsorbed, can be used for flow cytometry or for tissue staining. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining. 

This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The mouse adsorbed anti-rat IgG (H+L) products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross-react with mouse immunoglobulins. This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues.