- Affinity-purified, ultrapure, high affinity antibody
- Suitable for use with goat, sheep, and bovine IgG primary antibodies
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
- Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
- Optimally labeled with fluorescein to provide the brightest label for fluorescence microscopy
- Supplied in solution
- Excitation: 495 nm
- Emission: 515 nm
- Color: Green
|Country of Manufacture
||Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
|Color of Fluorescence
The rabbit anti-goat IgG antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).
Fluorescein Rabbit Anti-Goat IgG Antibody can be used for flow cytometry or for tissue staining. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining. Maximum excitation is at 495 nm and maximum emission is at 515 nm.
As well as recognising goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.
The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker solution (Cat. No. SP-5030) is recommended.