Fluorescein Maleimide is used to label free thiol groups with fluorescein. This fluorescent label can be directly visualized or used as a specific tag that can be detected with anti-fluroescein antibodies.
|Unit Size||12 mg|
|Country of Manufacture||United States|
|Target for Labeling||DNA, Oligonucleotides, PNA, Protein, RNA|
|Required Reactive Group||Thiol Group|
Fluorescein Maleimide is designed for fluoresceinating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. In the case of proteins, Fluorescein Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane).
For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag®, 5' EndTag™ or 3' EndTag™ nucleic acid labeling systems.
This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents. (Excitation maximum at 495 nm; emission maximum at 515 nm).