Fluorescein labeled, succinylated Wheat Germ Agglutinin (WGA)

Cat. No: FL-1021S
Price: $147.00
Unit Size: 5 mg

Overview

This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine.

Fluorescein labeled, succinylated WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.


Cat. No. FL-1021S-5
Unit Size 5 mg
Country of Manufacture United States
Applications Immunofluorescence, Glycobiology
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylglucosamine

More Information

This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.

Fluorescein labeled, succinylated WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

Protocols/Data Sheets/SDS