Succinylated Wheat Germ Agglutinin (WGA), Fluorescein

FL-1021S
SKU Unit Size Price Qty
FL-1021S-5 5 mg
$168.00

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Description

This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine.

Fluorescein labeled, succinylated WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.


Specifications

More Information
Unit Size 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C; Store frozen for long term storage
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylglucosamine

Documents

Technical Information

Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required