Fluorescein labeled Phaseolus Vulgaris Leucoagglutinin (PHA-L)

Cat. No: FL-1111
Price: $141.00
Unit Size: 2 mg

Overview

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins.  PHA-L, with four "L" type subunits, does not agglutinate red cell but is a potent mitogen.

Fluorescein labeled PHA-L has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Cat. No. FL-1111-2
Unit Size 2 mg
Country of Manufacture United States
Applications Immunofluorescence, Glycobiology
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Galactose, Complex Structures

More Information

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the E subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the L subunit (for leucoagglutinin). These subunits combine to produce five isolectins.

One of these isolectins has four E subunits and is designated PHA-E. PHA-E possesses strong hemagglutinating activity but is a poor mitogen. PHA-L, with four L type subunits, does not agglutinate red cells but is a potent mitogen. The other three isolectins, designated E3L1, E2L2, and E1L3, have erythroagglutinating and mitogenic activities proportional to the number of respective E or L subunits. We have termed the mixture of the five isolectins PHA (E+L).

Unconjugated PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing. After iontophoretic injection of unconjugated PHA-L, the approximate rate of anterograde transport is about 4-6 mm/day, with survival periods of over 18 days having been observed. Once transported, the unconjugated PHA-L is best visualized for light microscopy with antibody to the lectin, followed by the appropriate detection system and substrate. Fluorescent staining can also be used for a faster staining procedure.
 
Note: Do not use a fluorescently conjugated PHA-L to perform anterograde neuronal tracing. Only the unconjugated PHA-L (catalog # L-1110) is compatible.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Elution: 100 mM acetic acid

Safety Information

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