Fluorescein labeled Agaricus Bisporus Lectin (ABL)

Cat. No: FL-1421
Price: $136.00
Unit Size: 1 mg

Overview

Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits.  This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine.  Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms.  Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity.  ABL binds all blood groups and O-linked glycans of IgA subclasses.  Simple mono- and disaccharides are only marginally inhibitory.

Fluorescein labeled Agaricus bisporus lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Cat. No. FL-1421-1
Unit Size 1 mg
Country of Manufacture United States
Applications Immunofluorescence, Glycobiology
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Gal(b-1,3)GalNAc (T disaccharide)

More Information

Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits.  This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine.  Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms.  Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity.  ABL binds all blood groups and O-linked glycans of IgA subclasses.  Simple mono- and disaccharides are only marginally inhibitory.

Fluorescein labeled Agaricus bisporus lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Protocols/Data Sheets/SDS