Fluorescein Goat Anti-Mouse IgM, mu chain specific, can be used for flow cytometry or for tissue staining. This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins.
Features:
This mu chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains
Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining
Supplied in solution
Excitation: 495 nm
Emission: 515 nm
Color: Green
Specifications
More Information
Unit Size
0.5 mg
Applications
Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration
1 mg active conjugate/ml
Recommended Storage
2-8 °C
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission
510-520 nm
Maximum Excitation
490-500 nm
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.
This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies. This chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.
Powered by Bioz