Note: Requires thiol-reactive labeling reagent:
|Unit Size||1 kit|
|Country of Manufacture||United States|
|Target for Labeling||DNA, PNA, RNA|
The FastTag® Nucleic Acid Labeling System is the method of choice for incorporating labels at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor create its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.
DNA/RNA labeling is based on aryl azide chemistry in which the reagent, when exposed to heat or light, becomes activated and incorporates into the nucleic acid without base specificity. The nucleic acid labeling reaction can be carried out using a mercury vapor bulb (sun lamp), a UV lamp which produces light in the 350 nm to 370 nm range, a halogen lamp, a heating block, or a thermal cycler providing the investigator with great flexibility in experimental design.
A distinct advantage of the simple setup in each of these labeling options is the ease and economy of scaling up.
The FastTag® Nucleic Acid Labeling System utilizes a disulfide-containing universal linker that is incorporated into nucleic acid simply by using heat or light. Reducing the disulfide bond yields a free thiol group. A variety of thiol-reactive haptens, fluorochromes, affinity ligands or other markers can then be covalently bound to the nucleic acid via the FastTag reagent thiol group.
The FastTag® Labeling Kit contains the following reagents to label up to 250 μg of nucleic acid (or to carry out up to 50 labeling reactions):
Use this kit with any thiol-reactive label. Thiol-reactive label is not included in the kit and should be selected separately.
Detection by electron microscopy of a plasmid biotinylated with FastTag Biotin and then gold-labeled. The plasmid, complexed with loctosylated PEI, is taken up by an airway epithelial cell. Image kindly supplied by Drs. S. Grosse and I. Fajac, Faculté de Médecine Paris 5, Paris, France.
Detection in an airway epithelial cell of a plasmid biotinylated with FastTag Biotin and then labeled with rhodamine-conjugated streptavidin (red). The nuclear membrane is labeled with an anti-lamin A/C antibody and appears green. Image kindly supplied by Drs. S. Grosse and I. Fajac, Faculté de Médecine Paris 5, Paris, France.
A plasmid biotinylated with FastTag Biotin was complexed with a lactosylated polyethylenimine and instilled intranasally to mice. On this tissue slide, the plasmid labeled with rhodamine-conjugated streptavidin (red) is detected in epithelial cells of the bronchus and in the pulmonary cells surrounding it. Images kindly supplied by G. Thévenot and Dr. I. Fajac, Faculté de Médecine Paris 5, Paris, France.