The Lycopersicon Esculentum (tomato) lectin has been widely reported as an effective blood vessel marker forin vivovascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods.
Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below:
Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2)
Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112