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DyLight 594 labeled Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

Catalog Number: DL-1177

Availability: In stock

$130.00
Unit Size: 1 mg

Retina flat mount from mice, perfused with DyLight 594-LEL. The retina were mounted in Vectashield HardSet+DAPI.  Image courtesy of George W. Smith, Florida Atlantic University. Zoom

Overview

    Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

    DyLight® 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

    The excitation maximum is at 593 nm and the emission maximum is at 618 nm (red).

    Additional Information

    Catalog Number DL-1177
    Unit Size 1 mg
    Country of Manufacture United States
    Applications Immunofluorescence, Glycobiology
    Conjugate DyLight 594
    Color of Fluorescence Red
    Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine
Additional Info

    Details

    Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

    Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

    Texas Red® or DyLight® 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

    DyLight® 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

    Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

    • Excitation maximum:  593 nm
    • Emission maximum:  618 nm
    • Color:  Red

    Inhibiting/Eluting Sugar: Chitin Hydrolysate

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Protocols/Data Sheet/SDS