Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594

DL-1177
SKU Unit Size Price Qty
DL-1177-1 1 mg
$159.00

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Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight® 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  592 nm
  • Emission maximum:  617 nm
  • Color:  Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 592 nm
Maximum Emission 617 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 594
Color of Fluorescence Red
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

Can the biotinylated Lycopersicon Esculentum (tomato) lectin be used for in vivoperfusion studies to trace blood vessels in mice?

The Lycopersicon Esculentum (tomato) lectin has been widely reported as an effective blood vessel marker forin vivovascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® or DyLight® 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

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