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DyLight 594 Labeled Griffonia Simplicifolia Lectin I (GSL I) isolectin B4

Catalog Number: DL-1207

Availability: In stock

$125.00
Unit Size: 0.5 mg

Mouse tongue (formalin-fixed, paraffin-embedded) stained with DyLight 594-Labeled GSL 1, Isolectin B4 (red) and counterstained with DAPI (blue). Zoom

Overview

    GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.  This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. 

    DyLight® 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight® 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

    Excitation maximum is at 592 nm and emission maximum is at 617 nm (red).

    Additional Information

    Catalog Number DL-1207
    Unit Size 0.5 mg
    Country of Manufacture United States
    Applications Immunofluorescence, Glycobiology
    Conjugate DyLight 594
    Color of Fluorescence Red
    Sugar Specificity Galactose
Additional Info

    Details

    GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

    GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

    DyLight® 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight® 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

    Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

    • Excitation maximum:  592 nm
    • emission maximum:  617 nm
    • Color:  Red

    Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

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